This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wang, S P Articles by Holmes, K K Search for Related Content PubMed PubMed Citation Articles by Wang, S P Articles by Holmes, K K

 Previous Article  |  Next Article 

J Clin Microbiol. 1975 March; 1(3): 250-255

Simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens for use as a screening test for antibody.

ABSTRACT

A simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens has been devised as a screening test for antibody in human sera. The test differs from our standard procedure by amalgamating 15 different immunotypes into nine antigen pools, by using only three serum dilutions, and by dropping use of duplicate slides. The screening test could be performed on at least six times as many sera as the standard test for a given unit of effort. It was shown to have a sensitivity equal to the standard test and an ability to determine specific immunotype on the basis of antibody pattern (72%) only slightly reduced from the standard test (84%). The screening test was carried out on 876 patients from different population groups in the Seattle area. The tests showed that antibody was present frequently in persons attending venereal disease clinics (60%) and commonly (25%) in a group of adults without venereal disease. Nine percent of children under 15 years of age tested had antibody; the significance of this finding is unknown.

This article has been cited by other articles:

• Patton, D. L., Cosgrove Sweeney, Y. T., McCarthy, T. D., Hillier, S. L. (2006). Preclinical Safety and Efficacy Assessments of Dendrimer-Based (SPL7013) Microbicide Gel Formulations in a Nonhuman Primate Model.. Antimicrob. Agents Chemother. 50: 1696-1700 [Abstract] [Full Text]  
• Jones, C S, Maple, P A C, Andrews, N J, Paul, I D, Caul, E O (2003). Measurement of IgG antibodies to Chlamydia trachomatis by commercial enzyme immunoassays and immunofluorescence in sera from pregnant women and patients with infertility, pelvic inflammatory disease, ectopic pregnancy, and laboratory diagnosed Chlamydia psittaci/Chlamydia pneumoniae infection. J. Clin. Pathol. 56: 225-229 [Abstract] [Full Text]  
• Huhtinen, M, Laasila, K, Granfors, K, Puolakkainen, M, Seppala, I, Laasonen, L, Repo, H, Karma, A, Leirisalo-Repo, M (2002). Infectious background of patients with a history of acute anterior uveitis. Ann Rheum Dis 61: 1012-1016 [Abstract] [Full Text]  
• Marston, E. L., James, A. V., Parker, J. T., Hart, J. C., Brown, T. M., Messmer, T. O., Jue, D. L., Black, C. M., Carlone, G. M., Ades, E. W., Sampson, J. (2002). Newly Characterized Species-Specific Immunogenic Chlamydophila pneumoniae Peptide Reactive with Murine Monoclonal and Human Serum Antibodies. CVI 9: 446-452 [Abstract] [Full Text]  
• Stothard, D. R. (2001). Use of a Reverse Dot Blot Procedure To Identify the Presence of Multiple Serovars in Chlamydia trachomatis Urogenital Infection. J. Clin. Microbiol. 39: 2655-2659 [Abstract] [Full Text]  
• Huhtinen, M., Puolakkainen, M., Laasila, K., Sarvas, M., Karma, A., Leirisalo-Repo, M. (2001). Chlamydial Antibodies in Patients with Previous Acute Anterior Uveitis. IOVS 42: 1816-1819 [Abstract] [Full Text]  
• Verkooyen, R. P., Willemse, D., Hiep-van Casteren, S. C. A. M., Mousavi Joulandan, S. A., Snijder, R. J., van den Bosch, J. M. M., van Helden, H. P. T., Peeters, M. F., Verbrugh, H. A. (1998). Evaluation of PCR, Culture, and Serology for Diagnosis of Chlamydia pneumoniae Respiratory Infections. J. Clin. Microbiol. 36: 2301-2307 [Abstract] [Full Text]  
• Komaroff, A., Aronson, M., Pass, T., Ervin, C., Branch, W. Jr, Schachter, J (1983). Serologic evidence of chlamydial and mycoplasmal pharyngitis in adults. Science 222: 927-929 [Abstract]