This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rosett, W. Articles by Hodges, G. R. Search for Related Content PubMed PubMed Citation Articles by Rosett, W. Articles by Hodges, G. R.

Antimicrobial Activity of Heparin

² Medical Service and Research Service, Veterans Administration Medical Center, Kansas City, Missouri 64128
¹ Department of Medicine, University of Kansas College of Health Sciences, Kansas City, Kansas 66103

ABSTRACT

Single clinical isolates of eight species of microorganisms were incubated in solutions of heparin and brain heart infusion broth at various concentrations to determine the possible antibacterial effect of heparin. At heparin concentrations ranging from 12.5 to 400 U/ml, the effect on Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Staphylococcus aureus, and S. epidermidis varied with brain heart infusion broth concentrations of 1.2 to 10% and inocula of 10² to 10⁶ colony-forming units per ml; similar effects were not observed with Klebsiella pneumoniae, Enterobacter aerogenes, and Citrobacter spp. The minimal inhibitory concentrations of heparin for ten strains of each species were determined in 2.5% brain heart infusion broth with inocula of 10⁴ colony-forming units per ml. All 10 isolates of S. aureus and all 10 of S. epidermidis were inhibited by heparin concentrations of 125 to 500 U/ml. Three E. coli, four P. aeruginosa, and nine C. albicans strains were inhibited by 500 U of heparin per ml. None of the K. pneumoniae, E. aerogenes, Enterobacter cloacae, and Citrobacter spp. was inhibited by heparin at 500 U/ml. Heparin inhibition of S. aureus in 2.5% brain heart infusion broth-500 U of heparin per ml could be quantitatively overcome by addition of magnesium, calcium, or magnesium and calcium. These data suggest that the growth of microorganisms from heparin-containing material may be suppressed.

This article has been cited by other articles:

• Kim, Y. S., Jo, Y. Y., Chang, I. M., Toida, T., Park, Y., Linhardt, R. J. (1996). A New Glycosaminoglycan from the Giant African Snail Achatina fulica. J. Biol. Chem. 271: 11750-11755 [Abstract] [Full Text]