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J Clin Microbiol. 1980 September; 12(3): 447-450

Respiratory syncytial virus serology by a simplified enzyme-linked immunosorbent assay.

M C Steinhoff, C B Hall and K C Schnabel

ABSTRACT

A simplified enzyme-linked immunosorbent assay (ELISA) which utilized commercially available reagents was developed for respiratory syncytial virus (RSV)-specific immunoglobulin G. An analysis of the inherent variation of the assay allowed the setting of strict criteria for determining a significant change in RSV antibodies. The ELISA was more sensitive than the standard complement fixation or microneutralization tests in a carefully studied group of 32 RSV-infected adults. The ELISA correlated closely with complement fixation serological testing in 25 patients. The use of purified antigens might allow the development of a more sensitive ELISA.

J Clin Microbiol. 1980 September; 12(3): 447-450






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1980 by the American Society for Microbiology. All rights reserved.