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J Clin Microbiol. 1980 November; 12(5): 714-717

Improvements in the passive immune hemolysis test for assaying enterotoxigenic Escherichia coli.

A F de Castro, M B Serafim, J A Gomes and M S Gatti

ABSTRACT

The sensitivity of the passive immune hemolysis test for the detection of heat-labile enterotoxin from Escherichia coli was increased when the test was carried out with Veronal-buffered saline plus Ca2+ and Mg2+ as diluent, instead of phosphate-buffered saline. The performance of the test was further improved by using stationary cultures to which mitomycin C had been added at the end of the lag phase.

J Clin Microbiol. 1980 November; 12(5): 714-717






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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