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J Clin Microbiol. 1980 December; 12(6): 799-801

Preservation of nasopharyngeal smears for fluorescent antibody detection of Bordetella pertussis.

P P Harris, B Thomason and R M McKinney

ABSTRACT

Smears from throat and nasal washings seeded with Bordetella pertussis were either treated with aprotinin (a protease inhibitor) or fixed with 1 or 10% Formalin. These smears were stored at 23, 4, and -70 degrees C. Smears were removed and stained with a fluorescent antibody conjugate for B. pertussis at intervals during 22 days of storage. Results indicate that treatment of smears with 4 or 8 U of aprotinin per ml preserved fluorescent antibody staining qualities of B. pertussis for 22 days; fixation with either concentration of Formalin was unsatisfactory.

J Clin Microbiol. 1980 December; 12(6): 799-801






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1980 by the American Society for Microbiology. All rights reserved.