This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Murray, B E Articles by Echeverria, P Search for Related Content PubMed PubMed Citation Articles by Murray, B E Articles by Echeverria, P

 Previous Article  |  Next Article 

J Clin Microbiol. 1981 January; 13(1): 179-183

Toxin detection after storage or cultivation of enterotoxigenic with colicinogenic Escherichia coli: a possible mechanism for toxin-negative pools.

ABSTRACT

Of 100 non-enterotoxigenic Escherichia coli isolated from children with diarrhea in Bangkok, Thailand, 24 were found to produce colicin(s). Of these, 87% were active against one or more enterotoxigenic E. coli isolated from the same population. Storage of nine enterotoxigenic E. coli with known inhibitory colicin-producing E. coli in different proportions caused 51 of 96 pools to become negative in the suckling mouse assay (heat-stable toxin) and 17 of 52 to become negative in the Y-1 adrenal cell assay (heat-labile toxin). Cocultivation of the same strains, without prior storage, caused 12 of 96 pools to become negative for heat-stable toxin and 1 of 52 pools to become negative for heat-labile toxin. Storage or cultivation of E. coli in pools may cause negative results in the suckling mouse and Y-1 adrenal cell assays if any of the isolates in the pool produces colicin(s).