This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Murray, P R Articles by Niles, A C Search for Related Content PubMed PubMed Citation Articles by Murray, P R Articles by Niles, A C

 Previous Article  |  Next Article 

J Clin Microbiol. 1981 January; 13(1): 85-88

Detection of bacteriuria: manual screening test and early examination of agar plates.

ABSTRACT

We assessed two procedures for the initial processing of urine specimens: (i) manual screening of urine samples by inoculation into tryptic soy broth and incubation for 3 to 6 h, and (ii) examination of agar plates inoculated with urine and incubated for 4 to 6 h. Of the urine samples with greater than 10(5) colony-forming units per ml, 73.6 and 90.1% were detected with the broth cultures after 3 and 6 h of incubation, respectively. The organisms not detected at 6 h were Corynebacterium spp., Lactobacillus spp., and one of six isolates of yeasts. When inoculated plates were examined for growth, 53.3 and 80.0% of the specimens with greater than 10(5) colony-forming units per ml were detected after 4 and 6 h of incubation, respectively. Of the cultures with significant growth of Enterobacteriaceae or Pseudomonas spp., 95% were detected on the plates after 6 h of incubation.