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J Clin Microbiol. 1981 March; 13(3): 449-455

Enzyme-linked immunosorbent assay compared with immunoprecipitation for serotyping Neisseria meningitidis and its use in demonstrating serotype polymorphism.

E B Mackie, L A White, K N Brown and L E Bryan

ABSTRACT

Rabbit antisera generated against 11 prototype strains of group B Neisseria meningitidis were tritiated by using the enzyme-linked immunosorbent assay and the Ouchterlony immunoprecipitation technique. The relative insensitivity of the immunoprecipitation technique in detecting antibody compared with the enzyme-linked immunosorbent assay was demonstrated. Cross-reactions within the group and among the 11 serotypes were characterized with respect to established serological techniques. Serotype polymorphism among the strains of N. meningitidis was demonstrated with the enzyme-linked immunosorbent assay.

J Clin Microbiol. 1981 March; 13(3): 449-455






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1981 by the American Society for Microbiology. All rights reserved.