This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Guerrant, G O Articles by Moss, C W Search for Related Content PubMed PubMed Citation Articles by Guerrant, G O Articles by Moss, C W

 Previous Article  |  Next Article 

J Clin Microbiol. 1981 May; 13(5): 899-907

Gas-chromatographic analysis of mycolic acid cleavage products in mycobacteria.

ABSTRACT

Mycolic acids were detected in both reference strains and clinical isolates of mycobacteria using gas chromatography of fatty acid methyl esters prepared by acid methanolysis. The methyl esters were extracted with hexane, concentrated, and analyzed with a gas chromatograph by using two different injector temperatures. When the samples were analyzed at high injector temperatures of 300 to 350 degrees C, characteristic thermal cleavage products from mycolic acids, C22:0, C24:0, or C26:0 fatty acid methyl esters, were detected. When analyzed at injector temperatures of 235 degrees C or lower, the mycolic acids were heat stable and the characteristic methyl ester cleavage products were not observed.

This article has been cited by other articles:

• Bhatt, A., Fujiwara, N., Bhatt, K., Gurcha, S. S., Kremer, L., Chen, B., Chan, J., Porcelli, S. A., Kobayashi, K., Besra, G. S., Jacobs, W. R. Jr. (2007). Deletion of kasB in Mycobacterium tuberculosis causes loss of acid-fastness and subclinical latent tuberculosis in immunocompetent mice. Proc. Natl. Acad. Sci. USA 104: 5157-5162 [Abstract] [Full Text]  
• Singh, A., Gupta, R., Vishwakarma, R. A., Narayanan, P. R., Paramasivan, C. N., Ramanathan, V. D., Tyagi, A. K. (2005). Requirement of the mymA Operon for Appropriate Cell Wall Ultrastructure and Persistence of Mycobacterium tuberculosis in the Spleens of Guinea Pigs. J. Bacteriol. 187: 4173-4186 [Abstract] [Full Text]  
• Selvarangan, R., Wu, W.-K., Nguyen, T. T., Carlson, L. D. C., Wallis, C. K., Stiglich, S. K., Chen, Y.-C., Jost, K. C. Jr., Prentice, J. L., Wallace, R. J. Jr., Barrett, S. L. R., Cookson, B. T., Coyle, M. B. (2004). Characterization of a Novel Group of Mycobacteria and Proposal of Mycobacterium sherrisii sp. nov.. J. Clin. Microbiol. 42: 52-59 [Abstract] [Full Text]  
• Tortoli, E. (2003). Impact of Genotypic Studies on Mycobacterial Taxonomy: the New Mycobacteria of the 1990s. Clin. Microbiol. Rev. 16: 319-354 [Abstract] [Full Text]  
• Tortoli, E., Bartoloni, A., Bottger, E. C., Emler, S., Garzelli, C., Magliano, E., Mantella, A., Rastogi, N., Rindi, L., Scarparo, C., Urbano, P. (2001). Burden of Unidentifiable Mycobacteria in a Reference Laboratory. J. Clin. Microbiol. 39: 4058-4065 [Abstract] [Full Text]