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J Clin Microbiol. 1981 November; 14(5): 530-533

Extraction of group A streptococcal M protein with nitrous acid.

K Hafez, A M El Kholy and R R Facklam

ABSTRACT

Specific precipitating and opsonic neutralizing antigens were identified in dilute-nitrous acid extracts of group A streptococci. This was done by decreasing the temperature and time of exposure of the streptococcal cells to the nitrous acid. Ninety percent of 31 strains of group A streptococcal dilute-nitrous acid extracts formed precipitin lines of identity with conventional Lancefield extracts of the same group A streptococci.

J Clin Microbiol. 1981 November; 14(5): 530-533




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