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J Clin Microbiol. 1982 February; 15(2): 204-207

Production and characterization of a hybridoma-derived antibody to Blastomyces dermatitidis.

K D Young and H W Larsh

ABSTRACT

A hybridoma cell line was isolated which produced monoclonal antibody to one protein component of a yeast-phase cytoplasmic antigenic complex of Blastomyces dermatitidis. The immunoglobulin M antibody product was characterized by immunodiffusion, autoradiography of polyacrylamide gels, and cellulose acetate electrophoresis. By attaching the antibody to an affinity gel, one major protein band was identified by polyacrylamide gel electrophoresis as the antigen for which the antibody was specific.

J Clin Microbiol. 1982 February; 15(2): 204-207






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1982 by the American Society for Microbiology. All rights reserved.