JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zylber, L J
Right arrow Articles by Jordan, H V
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zylber, L J
Right arrow Articles by Jordan, H V
J Clin Microbiol. 1982 February; 15(2): 253-259

Development of a selective medium for detection and enumeration of Actinomyces viscosus and Actinomyces naeslundii in dental plaque.

L J Zylber and H V Jordan

ABSTRACT

A selective medium (CFAT) was developed for the detection and enumeration of Actinomyces viscosus and Actinomyces naeslundii in dental plaque. Neutral acriflavin and potassium tellurite were used in combination with the known selective agents cadmium and fluoride to eliminate most of the competing plaque flora. Composition of CFAT per liter was as follows: Trypticase soy broth (BBL Microbiology Systems), 30 g; glucose, 5 g; agar, 15 g' cadmium sulfate, 13 mg; sodium fluoride, 85 mg; neutral acriflavin, 1.20 mg; potassium tellurite, 2.50 mg; basic fuchsin, 1.25 mg; defibrinated sheep blood, 50 ml. A. viscosus reference strains of human origin grew on CFAT without reduction in numbers under an atmosphere of 90% air-10% CO2. Animal strains of A. viscosus were inhibited at the level of cadmium in CFAT. Two of six A. naeslundii strains did not grow on CFAT. Improved recovery of A. viscosus and A. naeslundii from dental plaque occurred on CFAT, as compared with two other selective media which contained either cadmium sulfate or sodium fluoride, respectively, as selective agents. CFAT was more selective with regard to much of the extraneous gram-positive flora. Bacterionemia, Neisseria, yeasts, and streptococci were virtually eliminated.

J Clin Microbiol. 1982 February; 15(2): 253-259




This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1982 by the American Society for Microbiology. All rights reserved.