This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Garcia, L S Articles by Shimizu, R Y Search for Related Content PubMed PubMed Citation Articles by Garcia, L S Articles by Shimizu, R Y

Comparison of indirect fluorescent-antibody amoebic serology with counterimmunoelectrophoresis and indirect hemagglutination amoebic serologies.

ABSTRACT

Patients ranged from those with no prior diagnosis of or suspected exposure to Entamoeba histolytica to those with proven amoebic liver abscesses (extraintestinal disease). A comparison of serologies from patients with proven and suspected amoebiasis or possible past exposure revealed good correlation between the indirect fluorescent antibody (IFA) procedure and the other methods used, counterimmunoelectrophoresis and indirect hemagglutination. Titers from patients with proven extraintestinal amoebiasis were in the expected high range previously reported by other authors. Patients with clinical histories suggestive of exposure to E. histolytica but no proven disease had lower titers which indicated possible background exposure. The IFA procedure provides a rapid method of antibody detection; results obtained on an emergency basis provide essential information in making the diagnosis of amoebic abscess, pyogenic abscess, or tumor. The IFA procedure is rapid, reliable reproducible, and relatively inexpensive to perform, provided a good source of antigen is consistently available.

This article has been cited by other articles:

• van Doorn, H. R., Hofwegen, H., Koelewijn, R., Gilis, H., Peek, R., Wetsteyn, J. C. F. M., van Genderen, P. J. J., Vervoort, T., van Gool, T. (2005). Use of Rapid Dipstick and Latex Agglutination Tests and Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Amebic Liver Abscess, Amebic Colitis, and Entamoeba histolytica Cyst Passage. J. Clin. Microbiol. 43: 4801-4806 [Abstract] [Full Text]  
• Tanyuksel, M., Petri, W. A. Jr. (2003). Laboratory Diagnosis of Amebiasis. Clin. Microbiol. Rev. 16: 713-729 [Abstract] [Full Text]