This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Maddison, S E Articles by Norman, L G Search for Related Content PubMed PubMed Citation Articles by Maddison, S E Articles by Norman, L G

Fractionation of Pneumocystis carinii antigens used in an enzyme-linked immunosorbent assay for antibodies and in the production of antiserum for detecting Pneumocystis carinii antigenemia.

ABSTRACT

Cyst-rich suspensions of Pneumocystis carinii were obtained by differential and gradient centrifugation from heavily infected rat lungs. After preparation of an aqueous-soluble extract of the cyst-rich material, the insoluble residue was extracted with 8 M urea. Small amounts of infected human lung tissue and uninfected rat and human lung were processed similarly. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that both human and rat infected lung extracts contained a large protein (greater than 200,000 daltons). This component was not present in extracts of uninfected lung. In addition, an HCl-soluble extract was prepared from the cyst-rich suspension from infected rat lung. The urea-extracted antigen was most reactive in an enzyme-linked immunosorbent assay. Rabbit antiserum against the HCl-soluble antigen detected circulating antigen in patients' sera in a counterimmunoelectrophoresis assay.

This article has been cited by other articles:

• Daly, K. R., Koch, J. V., Shire, N. J., Levin, L., Walzer, P. D. (2006). Human Immunodeficiency Virus-Infected Patients with Prior Pneumocystis Pneumonia Exhibit Increased Serologic Reactivity to Several Major Surface Glycoprotein Clones. CVI 13: 1071-1078 [Abstract] [Full Text]