This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Elder, B L Articles by Fives-Taylor, P M Search for Related Content PubMed PubMed Citation Articles by Elder, B L Articles by Fives-Taylor, P M

 Previous Article  |  Next Article 

J Clin Microbiol. 1982 July; 16(1): 141-144

Whole-bacterial cell enzyme-linked immunosorbent assay for Streptococcus sanguis fimbrial antigens.

ABSTRACT

A whole-bacterial cell enzyme-linked immunosorbent assay (bactELISA) was developed for detecting fimbrial antigens on Streptococcus sanguis. In this assay, S. sanguis cells were directly adhered to polystyrene or polyvinyl via drying. Use of the assay indicated that consistently high and uniform optical densities could be obtained from well to well. In addition, radioactive assaying indicated increased adsorption to the polystyrene wells over polyvinyl, suggesting that polystyrene may prove superior in the gram-positive bactELISA. Use of the bactELISA may prove valuable to both the clinical and research laboratory involved in the study of bacterial cell surface components or in the evaluation of antisera directed against bacterial antigens, which are difficult to prepare as purified derivatives.

This article has been cited by other articles:

• Wu, H., Zeng, M., Fives-Taylor, P. (2007). The Glycan Moieties and the N-Terminal Polypeptide Backbone of a Fimbria-Associated Adhesin, Fap1, Play Distinct Roles in the Biofilm Development of Streptococcus parasanguinis. Infect. Immun. 75: 2181-2188 [Abstract] [Full Text]  
• Wu, H., Bu, S., Newell, P., Chen, Q., Fives-Taylor, P. (2007). Two Gene Determinants Are Differentially Involved in the Biogenesis of Fap1 Precursors in Streptococcus parasanguis. J. Bacteriol. 189: 1390-1398 [Abstract] [Full Text]  
• Rose, J. E., Meyer, D. H., Fives-Taylor, P. M. (2003). Aae, an Autotransporter Involved in Adhesion of Actinobacillus actinomycetemcomitans to Epithelial Cells. Infect. Immun. 71: 2384-2393 [Abstract] [Full Text]  
• Chen, Q., Wu, H., Fives-Taylor, P. M. (2002). Construction of a Novel Transposon Mutagenesis System Useful in the Isolation of Streptococcus parasanguis Mutants Defective in Fap1 Glycosylation. Infect. Immun. 70: 6534-6540 [Abstract] [Full Text]  
• Froeliger, E. H., Fives-Taylor, P. (2001). Streptococcus parasanguis Fimbria-Associated Adhesin Fap1 Is Required for Biofilm Formation. Infect. Immun. 69: 2512-2519 [Abstract] [Full Text]  
• Oetjen, J., Fives-Taylor, P., Froeliger, E. (2001). Characterization of a Streptococcal Endopeptidase with Homology to Human Endothelin-Converting Enzyme. Infect. Immun. 69: 58-64 [Abstract] [Full Text]  
• Froeliger, E. H., Tomich, M., Fives-Taylor, P. (1999). Construction and Analysis of a Streptococcus parasanguis recA Mutant: Homologous Recombination Is Not Required for Adhesion in an In Vitro Tooth Surface Model. J. Bacteriol. 181: 63-67 [Abstract] [Full Text]