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J Clin Microbiol. 1982 August; 16(2): 406-407

Beta-lactamase testing of staphylococci in a commercial broth microdilution minimum inhibitory concentration system.

K A Horton, B R Jennings and V S Baselski

ABSTRACT

Beta-Lactamase testing of clinical isolates of staphylococci may be performed in commercial broth microdilution minimum inhibitory concentration plates, using a chromogenic cephalosporin reagent directly in a well containing a noninhibitory concentration of a semisynthetic penicillin which serves as an inducer. A total of 115 staphylococcal isolates tested in 0.25 to 0.50 microgram of methicillin per ml in Mueller-Hinton broth showed 100% correlation with beta-lactamase tests performed on Mueller-Hinton agar, using 1-microgram oxacillin disk as an inducer.

J Clin Microbiol. 1982 August; 16(2): 406-407






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 1982 by the American Society for Microbiology. All rights reserved.