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J Clin Microbiol. 1982 October; 16(4): 772-774

Rapid, modified oxidase test for oxidase-variable bacterial isolates.

J J Tarrand and D H Gröschel

ABSTRACT

A modified oxidase reagent, 1% tetramethyl-p-phenylenediamine in dimethyl sulfoxide, proved superior to the routinely used 1% aqueous tetramethyl-p-phenylenediamine dihydrochloride in detecting weakly oxidase-positive gram-negative bacteria after 24 h of growth on agar media (40 of 40 positive versus 22 of 40 positive). The bacterial inoculum was obtained with a cotton-tipped swab instead of a loop or wooden applicator, and the reaction required less than 15 s.

J Clin Microbiol. 1982 October; 16(4): 772-774




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