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J Clin Microbiol. 1982 December; 16(6): 1123-1126

Demonstration of age-dependent capsular material on Pasteurella haemolytica serotype 1.

R E Corstvet, M J Gentry, P R Newman, J A Rummage and A W Confer

ABSTRACT

Extracellular capsular material was demonstrated on early log-phase cells of Pasteurella haemolytica serotype 1 by the fluorescent-antibody and several capsular staining techniques. The presence of this material was shown to be age dependent. Wide capsules were demonstrable on cells from 2- to 12-h cultures, whereas cells from 16- to 22-h cultures had very little cell-associated capsular material. The Maneval technique most clearly demonstrated the presence of capsules on cells from young (6-h) cultures when compared with other capsule staining techniques.

J Clin Microbiol. 1982 December; 16(6): 1123-1126






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