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Quality Control Limits for the Agar Overlay Disk Diffusion Antimicrobial Susceptibility Test

Arthur L. Barry and Robert E. Badal

¹ Clinical Microbiology Laboratories, University of California, Davis, Medical Center, Sacramento, California 95817

ABSTRACT

Replicate control tests were performed with the agar overlay and Kirby-Bauer techniques, using two sources of Mueller-Hinton agar. Mean zones observed with the two methods differed by 1 to 3 mm. Despite the minor differences between methods, National Committee for Clinical Laboratory Standards quality control limits which were established for the Kirby-Bauer method could also be used for the agar overlay technique.

Present address: The Clinical Microbiology Institute, Tualatin, OR 97062.