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J Clin Microbiol. 1982 December; 16(6): 1157-1159

Methods for distinguishing gram-positive from gram-negative bacteria.

G M Carlone, M J Valadez and M J Pickett

ABSTRACT

Lysis by KOH and hydrolysis of L-alanine-4-nitroanilide were compared with the Gram reaction of aerobic, microaerophilic, and anaerobic bacteria. Both tests correlated well with the Gram reaction with nonfermentative bacilli and Bacillus species, whereas they did not correlate with nonsporulating anaerobes. Only campylobacteria were KOH positive and L-alanine-4-nitroanilide and gram negative.

J Clin Microbiol. 1982 December; 16(6): 1157-1159






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