This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Phillips, A P Articles by Broster, M G Search for Related Content PubMed PubMed Citation Articles by Phillips, A P Articles by Broster, M G

Differentiation between spores of Bacillus anthracis and Bacillus cereus by a quantitative immunofluorescence technique.

ABSTRACT

A quantitative immunofluorescence assay based on fiber optic microscopy was used to measure the reaction of formalized spores of Bacillus anthracis and Bacillus cereus isolates with fluorescein conjugates prepared by hyperimmunization with B. anthracis Vollum spores. The spores of 11 of the 20 B. cereus strains reacted with the anti-anthrax conjugate to such an extent that they were indistinguishable from the spores of the several B. anthracis isolates tested. However, absorption of the conjugate with spores of B. cereus NCTC 8035 and B. cereus NCTC 10320 greatly reduced the cross-reaction with the B. cereus preparations so that the mean specific fluorescence of samples of B. cereus spores was in no case higher than 14% of the fluorescence of a reference B. anthracis Vollum preparation.

This article has been cited by other articles:

• Goossens, P. L., Sylvestre, P., Mock, M. (2007). Of spore opsonization and passive protection against anthrax. Microbiology 153: 301-302 [Full Text]  
• Enkhtuya, J., Makino, S.-i., Uchida, I., Kawamoto, K. (2007). In reply. Microbiology 153: 302-304 [Full Text]