This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Doern, G V Articles by Parker, D S Search for Related Content PubMed PubMed Citation Articles by Doern, G V Articles by Parker, D S

Species identification and biotyping of staphylococci by the API staph-ident system.

ABSTRACT

The API Staph-Ident system, a commercially available biochemical and chromogenic substrate micromethod, was evaluated as a means for identifying the species and determining the biotypes of human strains of staphylococci routinely encountered in a clinical microbiology laboratory. The species identity of 152 of 188 (80.9%) unique clinical isolates of staphylococci was correctly predicted by this method after 5 h of incubation according to the recommendations of the manufacturer. When results were determined after 24 h of incubation, the overall accuracy of this procedure was 90.4%. The API Staph-Ident system was not an adequate procedure for assessing strain biotypes since the patterns of biochemical reactivity observed with 53 of 54 (98.2%) unique isolates of Staphylococcus aureus were identical. Similarly, 58 of 62 (93.6%) different strains of S. epidermidis yielded the same biochemical profile.

This article has been cited by other articles:

• Kawamura, Y., Hou, X.-G., Sultana, F., Hirose, K., Miyake, M., Shu, S.-E., Ezaki, T. (1998). Distribution of Staphylococcus Species among Human Clinical Specimens and Emended Description of Staphylococcus caprae. J. Clin. Microbiol. 36: 2038-2042 [Abstract] [Full Text]  
• Bascomb, S., Manafi, M. (1998). Use of Enzyme Tests in Characterization and Identification of Aerobic and Facultatively Anaerobic Gram-Positive Cocci. Clin. Microbiol. Rev. 11: 318-340 [Abstract] [Full Text]