This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hubbard, W A Articles by McClatchey, K D Search for Related Content PubMed PubMed Citation Articles by Hubbard, W A Articles by McClatchey, K D

Comparison of the B-D urine culture kit with a standard culture method and with the MS-2.

ABSTRACT

A total of 100 random clinical urine specimens were divided and used to compare Becton, Dickinson & Co. B-D Urine Culture Kit-treated urine (BDU) with refrigerated urine (RU). Each RU-BDU pair was cultured with the standard calibrated loop-plate method and also screened with the Abbott Laboratories MS-2 system at 0, 5, and 24 h. An additional 158 positive urine specimens containing greater than 10(5) CFU/ml were tested by standard culturing and MS-2 at 0 and 24 h. In addition, growth curves were run for Escherichia coli containing RU-BDU pairs at 0 and 24 h, and MS-2 detection time frequencies were analyzed. Culture results showed few changes in colony counts at 0 and 5 h for RU and BDU specimens. At 24 h, there were decreased colony counts for 7.0% of RU and 17.7% of BDU specimens. MS-2 results showed detection rates at 0 h of 100% for RU and 82.9% for BDU specimens and at 24 h of 91.1% for RU and 63.3% for BDU specimens. The organisms most frequently missed at 24 h were E. coli, Pseudomonas aeruginosa, and Staphylococcus epidermidis. Growth curve analysis showed a 1-h delay of logarithmic growth in BDU specimens. MS-2 detection time analysis showed delayed positive reports owing to be extended detection time for BDU specimens. The B-D Urine Culture Kit is a convenient transport system; however, some difficulty in recovering organisms at 24 h with the standard culture method and increased difficulty with the MS-2 system were recognized.