This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mittal, K R Articles by Lariviere, S Search for Related Content PubMed PubMed Citation Articles by Mittal, K R Articles by Lariviere, S

Determination of antigenic specificity and relationship among Haemophilus pleuropneumoniae serotypes by an indirect hemagglutination test.

ABSTRACT

Serological properties of antigens extracted from strains of Haemophilus pleuropneumoniae belonging to seven different serotypes were investigated. Antisera were prepared in rabbits against Formalin-treated whole cell suspensions as well as autoclaved cell suspensions. Saline and heat extracts and their alcohol precipitate antigens of H. pleuropneumoniae were used in the indirect hemagglutination test. All the antigens used were easily adsorbed directly onto sheep erythrocytes. Saline extract antigen showed maximum type specificity. Heating of the whole cell suspension revealed the cross-reactive minor antigenic determinants. Thus, the heat extract preparations had both type-specific and species-specific antigens. It is suggested that the indirect hemagglutination test may be useful for both serotyping and serodiagnosis of H. pleuropneumoniae infections in pigs.

This article has been cited by other articles:

• Tadjine, M., Mittal, K. R., Bourdon, S., Gottschalk, M. (2004). Development of a New Serological Test for Serotyping Haemophilus parasuis Isolates and Determination of Their Prevalence in North America. J. Clin. Microbiol. 42: 839-840 [Abstract] [Full Text]  
• Del Rio, M. L., Gutierrez, C.B., Rodriguez Ferri, E. F. (2003). Value of Indirect Hemagglutination and Coagglutination Tests for Serotyping Haemophilus parasuis. J. Clin. Microbiol. 41: 880-882 [Abstract] [Full Text]