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J Clin Microbiol. 1983 May; 17(5): 898-905

Detection of antibody to Staphylococcus aureus teichoic acid by enzyme-linked immunosorbent assay.

J K Yamada, C B Inderlied and R K Porschen

ABSTRACT

A sensitive, specific, and rapid enzyme-linked immunosorbent assay has been developed for the detection of immunoglobulin G to Staphylococcus aureus teichoic acid in human sera. Detection of S. aureus teichoic acid antibody is at least 800 times more sensitive than a double diffusion in gel assay, and positive titers of 1:25,600 and greater were observed with this assay. Results with the enzyme-linked immunosorbent assay can be obtained within 3.5 h by using antigen-coated cuvettes. Quantitation of S. aureus teichoic acid antibody by this enzyme-linked immunosorbent assay may be useful in the initial as well as the follow-up diagnosis of serious S. aureus infections.

J Clin Microbiol. 1983 May; 17(5): 898-905






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 1983 by the American Society for Microbiology. All rights reserved.