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J Clin Microbiol. 1983 May; 17(5): 928-930

Simple method for preparation of antibody-enzyme conjugates for enzyme-linked immunosorbent assays.

P J De Jong

ABSTRACT

Mixing suspensions of rabbit antibody for staphylococcal enterotoxin A and commercial anti-rabbit immunoglobulin G-alkaline phosphatase conjugate produced a complex conjugate suitable for use as the second antibody in a conventional enzyme-linked immunosorbent assay. The method was useful for detecting 1 ng of staphylococcal enterotoxin A per ml.

J Clin Microbiol. 1983 May; 17(5): 928-930






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1983 by the American Society for Microbiology. All rights reserved.