This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brayton, P R Articles by Colwell, R R Search for Related Content PubMed PubMed Citation Articles by Brayton, P R Articles by Colwell, R R

New selective plating medium for isolation of Vibrio vulnificus biogroup 1.

ABSTRACT

A new plating medium (VV agar) has been developed as an alternative to thiosulfate-citrate-bile salts-sucrose (TCBS) agar for the isolation of Vibrio vulnificus. Salicin (2% wt/vol) is employed as the source of carbohydrate, with potassium tellurite (0.0005% wt/vol), crystal violet (0.00015% wt/vol), oxgall (0.8% wt/vol), and a pH of 8.6 to inhibit growth of gram-positive and gram-negative organisms other than V. vulnificus. Because strains of V. vulnificus do not strongly ferment salicin in VV agar, a pH indicator has not been included in the medium. Growth of V. vulnificus appears on VV agar as large grey colonies with black centers. Other non-Vibrio strains which grow on the medium produce smaller colonies and fail to take up tellurite. VV agar has proved to be more effective than TCBS agar in inhibiting members of the Enterobacteriaceae as well as gram-positive cocci. Only Vibrio strains capable of utilizing salicin grow well on VV agar. Recovery and growth of V. vulnificus are superior on VV agar, compared with TCBS agar.

This article has been cited by other articles:

• Kim, J.-S., Sung, M.-H., Kho, D.-H., Lee, J. K. (2005). Induction of Manganese-Containing Superoxide Dismutase Is Required for Acid Tolerance in Vibrio vulnificus. J. Bacteriol. 187: 5984-5995 [Abstract] [Full Text]  
• Cerdà-Cuéllar, M., Jofre, J., Blanch, A. R. (2000). A Selective Medium and a Specific Probe for Detection of Vibrio vulnificus. Appl. Environ. Microbiol. 66: 855-859 [Abstract] [Full Text]  
• Høi, L., Dalsgaard, I., Dalsgaard, A. (1998). Improved Isolation of Vibrio vulnificus from Seawater and Sediment with Cellobiose-Colistin Agar. Appl. Environ. Microbiol. 64: 1721-1724 [Abstract] [Full Text]