This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Turunen, H J Articles by Saari, K M Search for Related Content PubMed PubMed Citation Articles by Turunen, H J Articles by Saari, K M

Demonstration of intraocular synthesis of immunoglobulin G toxoplasma antibodies for specific diagnosis of toxoplasmic chorioretinitis by enzyme immunoassay.

ABSTRACT

By using a modified enzyme immunoassay we have conducted a detailed study on the intraocular synthesis of toxoplasma antibodies during ocular toxoplasmosis. In control patients operated on for cataracts, the ratio of immunoglobulin G (IgG) toxoplasma antibodies in serum to those in aqueous humor was more than 100. In eight of the nine patients with clinically diagnosed toxoplasmic chorioretinitis, the ratio ranged between 6 and 56. In five patients with some other type of uveitis, the ratio was comparable with that in controls. Parallel determinations of mumps IgG antibody ratios were carried out to prove that the increased intraocular toxoplasma antibody levels were not a result of increased diffusion or polyclonal antibody stimulation. In all patients, mumps antibody ratios were within normal range. We also tested the samples of aqueous humor for IgM and IgA antibodies, but the antibodies in the aqueous humor were found to be exclusively IgG. Our results indicate that IgG-class anti-toxoplasma antibodies are produced locally within the eye in cases of toxoplasmic chorioretinitis. The determination of these antibodies can offer a valuable aid to make a specific etiological diagnosis of ocular toxoplasmosis.

This article has been cited by other articles:

• Lu, F., Huang, S., Kasper, L. H. (2004). CD4+ T Cells in the Pathogenesis of Murine Ocular Toxoplasmosis. Infect. Immun. 72: 4966-4972 [Abstract] [Full Text]  
• Simon, A., Labalette, P., Ordinaire, I., Frealle, E., Dei-Cas, E., Camus, D., Delhaes, L. (2004). Use of Fluorescence Resonance Energy Transfer Hybridization Probes To Evaluate Quantitative Real-Time PCR for Diagnosis of Ocular Toxoplasmosis. J. Clin. Microbiol. 42: 3681-3685 [Abstract] [Full Text]  
• Villard, O., Filisetti, D., Roch-Deries, F., Garweg, J., Flament, J., Candolfi, E. (2003). Comparison of Enzyme-Linked Immunosorbent Assay, Immunoblotting, and PCR for Diagnosis of Toxoplasmic Chorioretinitis. J. Clin. Microbiol. 41: 3537-3541 [Abstract] [Full Text]  
• Klaren, V. N. A., Peek, R. (2001). Evidence for a Compartmentalized B Cell Response as Characterized by IgG Epitope Specificity in Human Ocular Toxoplasmosis. J. Immunol. 167: 6263-6269 [Abstract] [Full Text]  
• Miller, D., Davis, J., Rosa, R., Diaz, M., Perez, E. (2000). Utility of Tissue Culture for Detection of Toxoplasma gondii in Vitreous Humor of Patients Diagnosed with Toxoplasmic Retinochoroiditis. J. Clin. Microbiol. 38: 3840-3842 [Abstract] [Full Text]  
• Garweg, J. G., Jacquier, P., Boehnke, M. (2000). Early Aqueous Humor Analysis in Patients with Human Ocular Toxoplasmosis. J. Clin. Microbiol. 38: 996-1001 [Abstract] [Full Text]