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J Clin Microbiol. 1983 October; 18(4): 968-971

Increased immunofluorescent staining of rabies-infected, formalin-fixed brain tissue after pepsin and trypsin digestion.

F L Reid, N H Hall, J S Smith and G M Baer

ABSTRACT

This study was undertaken to evaluate the sensitivity of the direct immunofluorescence test on Formalin-fixed, trypsin-digested, rabies-infected brain tissue. Our results suggest that the optimal unmasking of rabies antigenic sites is obtained by using a double enzyme digestion with pepsin and trypsin in lieu of only trypsin.

J Clin Microbiol. 1983 October; 18(4): 968-971






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1983 by the American Society for Microbiology. All rights reserved.