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J Clin Microbiol. 1984 March; 19(3): 383-387

Diagnostic value of interactions between members of the family Neisseriaceae and lectins.

ABSTRACT

The lectin slide agglutination test for Neisseria gonorrhoeae has been modified and improved. Results show that wheat germ agglutinin and soybean lectin agglutinate 100% (193 of 193 tested) of clinical isolates of N. gonorrhoeae. Lectin-reactive meningococci can be readily identified by the hydrolysis of gamma-glutamyl-beta-naphthylamide. Branhamella catarrhalis, Neisseria lactamica, Neisseria sicca, Neisseria subflava, Neisseria perflava, and meningococcal serogroups A, B, C, X, Y, and Z do not interfere with the positive identification of N. gonorrhoeae. The frequently encountered problem of autoagglutination of members of the family Neisseriaceae may be circumvented by a short treatment of cellular suspensions with DNase. Based on agglutination assays, the enzyme treatment did not result in a loss of wheat germ agglutinin receptors from the bacteria. The lectin agglutination test, coupled with the gamma-glutamyl aminopeptidase assay, is proposed as a rapid and accurate means of identifying clinical isolates of gonococci.

This article has been cited by other articles:

• ANNUK, H., HYNES, S. O., HIRMO, S., MIKELSAAR, M., WADSTROM, T. (2001). Characterisation and differentiation of lactobacilli by lectin typing. J Med Microbiol 50: 1069-1074 [Abstract] [Full Text]