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J Clin Microbiol. 1985 January; 21(1): 138-139

Serological analysis of dermatophyte isolates with monoclonal antibodies produced against Microsporum canis.

L Polonelli and G Morace

ABSTRACT

Hybridoma cells produced by fusing myeloma cells with spleen cells from mice immunized with a soluble antigen of Microsporum canis yielded 30 antibody-producing clones. Six of these clones, propagated as ascites tumors in mice, showed two different types of monoclonal antibodies. The type 1 monoclonal antibody reacted with 17 heterologous and 10 homologous dermatophyte antigens. Type 2 monoclonal antibodies were unable to precipitate three antigens from different isolates of M. canis, thus suggesting the occurrence of different serotypes within the species.

J Clin Microbiol. 1985 January; 21(1): 138-139






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