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J Clin Microbiol. 1985 April; 21(4): 643-644

Evaluation of two immunofluorescence assays with monoclonal antibodies for typing of herpes simplex virus.

E M Swierkosz, M Q Arens, R R Schmidt and T Armstrong

ABSTRACT

An indirect immunofluorescence assay and a direct immunofluorescence assay were evaluated for typing clinical isolates of herpes simplex virus (HSV). The indirect immunofluorescence assay (Electro-Nucleonics, Inc.) correctly identified 16 HSV type 2 (HSV-2) isolates, but failed to identify 4 of 14 HSV-1 isolates because of background fluorescence and instability of reagents. Forty-nine HSV-1 isolates were correctly typed by direct immunofluorescence assay (Kallestad Laboratories, Inc.), but 1 of 39 HSV-2 isolates did not react with the HSV-2 type-specific antibody conjugate.

J Clin Microbiol. 1985 April; 21(4): 643-644






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1985 by the American Society for Microbiology. All rights reserved.