This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Reimer, L G Articles by Reller, L B Search for Related Content PubMed PubMed Citation Articles by Reimer, L G Articles by Reller, L B

 Previous Article  |  Next Article 

J Clin Microbiol. 1985 May; 21(5): 686-688

Effect of sodium polyanetholesulfonate and gelatin on the recovery of Gardnerella vaginalis from blood culture media.

ABSTRACT

Sodium polyanetholesulfonate (SPS) is used as a routine supplement to blood culture media to enhance recovery of microorganisms, but it inhibits the growth of Peptostreptococcus anaerobius, Neisseria meningitidis, Neisseria gonorrhoeae, and Streptobacillus moniliformis. Comparative clinical blood culture studies at the University of Colorado Hospital suggested that SPS also inhibits the growth of Gardnerella vaginalis. We inoculated 16 blood culture isolates of G. vaginalis into 11 blood culture media containing SPS or sodium amylosulfate, with and without gelatin. In the absence of gelatin, only brain heart infusion and thiol broths with SPS supported the growth of more than five strains of G. vaginalis, whereas all media except Bactec 6B and 7C and brucella broths recovered most isolates with SPS and gelatin or with sodium amylosulfate alone. We conclude that SPS inhibits the growth of G. vaginalis in blood culture media but that this inhibition is medium dependent and can be overcome by supplementation of most media with gelatin.

This article has been cited by other articles:

• Pien, B. C., Mirrett, S., Crews, B. R., Reller, L. B., Woods, C. W. (2007). Controlled Clinical Comparison of BacT/ALERT Standard Aerobic and Standard Anaerobic Blood Culture Bottles Inoculated Directly or after Transport in Sodium Polyanethol Sulfonate Tubes. J. Clin. Microbiol. 45: 1357-1359 [Abstract] [Full Text]