This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bartus, H Articles by Zajac, I Search for Related Content PubMed PubMed Citation Articles by Bartus, H Articles by Zajac, I

 Previous Article  |  Next Article 

J Clin Microbiol. 1985 June; 21(6): 951-954

Indications that the erythrocyte receptor involved in enterotoxigenic Escherichia coli attachment is a sialoglycoconjugate.

ABSTRACT

A reverse hemagglutination assay was used to study adherence to human erythrocytes by Escherichia coli H10407, which possesses colonization factor antigen I. Pretreatment of erythrocytes with trypsin, chymotrypsin, papain, protease, and neuraminidase completely abolished attachment reactivity. In addition, the hemagglutination reaction was prevented by the presence of urea and guanidine. In contrast, the lipases, nucleotide hydrolases, exoglycosidases, and reagents affecting disulfide or sulfhydryl moieties did not alter receptor reactivity. Glycoconjugates containing sialic acid inhibited the hemagglutination reaction. Furthermore, a sialoglycoprotein isolated from the erythrocyte membrane inhibited the hemagglutination reaction. Collectively, these data indicate that the erythrocyte receptor responsible for attachment by E. coli possessing colonization factor antigen I is a sialoglycoconjugate.

This article has been cited by other articles:

• Li, Y.-F., Poole, S., Rasulova, F., McVeigh, A. L., Savarino, S. J., Xia, D. (2007). A Receptor-binding Site as Revealed by the Crystal Structure of CfaE, the Colonization Factor Antigen I Fimbrial Adhesin of Enterotoxigenic Escherichia coli. J. Biol. Chem. 282: 23970-23980 [Abstract] [Full Text]  
• Jansson, L., Tobias, J., Lebens, M., Svennerholm, A.-M., Teneberg, S. (2006). The Major Subunit, CfaB, of Colonization Factor Antigen I from Enterotoxigenic Escherichia coli Is a Glycosphingolipid Binding Protein.. Infect. Immun. 74: 3488-3497 [Abstract] [Full Text]