This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by de Repentigny, L Articles by Reiss, E Search for Related Content PubMed PubMed Citation Articles by de Repentigny, L Articles by Reiss, E

 Previous Article  |  Next Article 

J Clin Microbiol. 1985 June; 21(6): 972-979

Comparison of enzyme immunoassay and gas-liquid chromatography for the rapid diagnosis of invasive candidiasis in cancer patients.

ABSTRACT

Three proposed quantitative markers for candidiasis, arabinitol, mannose, and mannan in serum, are compared in 50 normal blood donors and 38 high-risk patients, 23 with and 15 without invasive candidiasis. Arabinitol concentrations in serum, the arabinitol/creatinine ratio, and mannose concentrations in serum were significantly greater in the 15 patients without candidiasis than in the normal blood donors (P less than 0.05). The sensitivities and specificities were 26 and 87% for arabinitol, 13 and 93% for the arabinitol/creatinine ratio, and 39 and 87% for mannose. On the other hand, mannan concentrations in serum were less than 1 ng/ml in normal blood donors and patients without candidiasis (P = 0.344), and the sensitivity and specificity were 65 and 100%, respectively. Of 23 patients with proven or probable candidiasis, 16 had mannan levels in serum greater than the mean + 2 standard deviations (0.46 ng/ml) for the 15 controls. In 16 patients with invasive candidiasis and positive blood cultures for the Candida spp., only 13 had elevated levels of at least one of the three markers. The arabinitol/creatinine ratio, the mannose level, and the mannan level became elevated an average of 4 days before, 1 day before, and on the same day that the blood cultures were drawn, respectively. Conversely, mannan was detected in the sera of six of seven patients with invasive candidiasis and negative blood cultures. We conclude that the best approach to diagnosing invasive candidiasis involves obtaining blood cultures and carrying out serial assays for mannan in serum.

This article has been cited by other articles:

• Kosonen, J., Rantala, A., Little, C. H., Lintu, P., Harjamaki, P.-R., Georgiou, G. M., Cone, R. E., Savolainen, J. (2006). Increased Levels of Candida albicans Mannan-Specific T-Cell-Derived Antigen Binding Molecules in Patients with Invasive Candidiasis. CVI 13: 467-474 [Abstract] [Full Text]  
• Verduyn Lunel, F. M., Voss, A., Kuijper, E. J., Gelinck, L. B. S., Hoogerbrugge, P. M., Liem, K. L., Kullberg, B. J., Verweij, P. E. (2004). Detection of the Candida Antigen Mannan in Cerebrospinal Fluid Specimens from Patients Suspected of Having Candida Meningitis. J. Clin. Microbiol. 42: 867-870 [Abstract] [Full Text]  
• Yeo, S. F., Wong, B. (2002). Current Status of Nonculture Methods for Diagnosis of Invasive Fungal Infections. Clin. Microbiol. Rev. 15: 465-484 [Abstract] [Full Text]  
• Chang, H. C., Leaw, S. N., Huang, A. H., Wu, T. L., Chang, T. C. (2001). Rapid Identification of Yeasts in Positive Blood Cultures by a Multiplex PCR Method. J. Clin. Microbiol. 39: 3466-3471 [Abstract] [Full Text]  
• El Moudni, B., Rodier, M.-H., Daniault, G., Jacquemin, J. L. (1998). Improved Immunodiagnosis of Human Candidiasis by an Enzyme-Linked Immunosorbent Assay Using a Candida albicans 52-Kilodalton Metallopeptidase. CVI 5: 823-825 [Abstract] [Full Text]