This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Campos, J M Articles by Charilaou, C C Search for Related Content PubMed PubMed Citation Articles by Campos, J M Articles by Charilaou, C C

 Previous Article  |  Next Article 

J Clin Microbiol. 1985 August; 22(2): 145-148

Evaluation of Detect-A-Strep and the Culturette Ten-Minute Strep ID kits for detection of group A streptococcal antigen in oropharyngeal swabs from children.

ABSTRACT

The Detect-A-Strep kit (DAS) (Antibodies Inc.) and the Culturette Brand 10-Minute Group A Strep ID kit (CBTMSI) (Marion Scientific) were compared with a sensitive culture method for detection of group A streptococci in oropharyngeal swabs from children. Specimens from 953 children ranging in age from 4 months to 18 years were inoculated to selective blood agar plates containing sulfamethoxazole-trimethoprim. Culture plates were incubated anaerobically for the first overnight period and aerobically in the presence of 10% CO2 for the second overnight period. The same specimens were tested within 24 h by DAS (phase 1) or CBTMSI (phase 2). One hundred thirty-five of 538 specimens (25.1%) tested by DAS and 150 of 415 specimens (36.1%) tested by CBTMSI were culture positive. DAS detected 87 of 135 (64.4%) and CBTMSI detected 93 of 150 (62.0%) culture-positive specimens. The specificities of DAS and CBTMSI were 96.5 and 99.6%, respectively. The predictive values of positive and negative results were 86.1 and 89.0% for DAS and 98.9 and 82.2% for CBTMSI. Because a reliable distinction between infected patients and carrier state patients cannot be made, we conclude that neither DAS nor CBTMSI is sufficiently sensitive to replace our culture method.

This article has been cited by other articles:

• Bourbeau, P. P. (2003). Role of the Microbiology Laboratory in Diagnosis and Management of Pharyngitis. J. Clin. Microbiol. 41: 3467-3472 [Full Text]  
• Deneef, P. (1987). Evaluating Rapid Tests for Streptococcal Pharyngitis: The Apparent Accuracy of a Diagnostic Test When There Are Errors in the Standard of Comparison. Med Decis Making 7: 92-96 [Abstract]