This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gary, G W Articles by Anderson, L J Search for Related Content PubMed PubMed Citation Articles by Gary, G W, Jr Articles by Anderson, L J

 Previous Article  |  Next Article 

J Clin Microbiol. 1985 August; 22(2): 274-278

Detection of Norwalk virus antibodies and antigen with a biotin-avidin immunoassay.

ABSTRACT

Biotin-avidin immunoassays (BAIs) were developed to detect Norwalk virus antigen and to measure Norwalk virus antibody. The BAI detected Norwalk virus infections by a fourfold titer rise in antibody in sera or by antigen in stool, with a sensitivity similar to or greater than that of the radioimmunoassay (RIA), and the BAI appeared to be more sensitive than the RIA for detecting antibody in single serum specimens. The BAI antigen test detected Norwalk antigen in all stools that were positive by the RIA, and in two stools negative by the RIA. Of 106 serum pairs tested by both the RIA and BAI, 94 demonstrated seroconversion, or lack of seroconversion, in both assays; 12 gave discordant results. Titers by the BAI generally exceeded those by the RIA by two- or fourfold. The BAI had advantages over the RIA in that it had added sensitivity in the detection of Norwalk antibody, was less time consuming, did not require frequent labeling of antibody, and did not have the problems associated with the use of isotopes.

This article has been cited by other articles:

• Atmar, R. L., Estes, M. K. (2001). Diagnosis of Noncultivatable Gastroenteritis Viruses, the Human Caliciviruses. Clin. Microbiol. Rev. 14: 15-37 [Abstract] [Full Text]  
• Maguire, A. J., Green, J., Brown, D. W. G., Desselberger, U., Gray, J. J. (1999). Molecular Epidemiology of Outbreaks of Gastroenteritis Associated with Small Round-Structured Viruses in East Anglia, United Kingdom, During the 1996-1997 Season. J. Clin. Microbiol. 37: 81-89 [Abstract] [Full Text]  
• Nakata, S., Honma, S., Numata, K., Kogawa, K., Ukae, S., Adachi, N., Jiang, X., Estes, M. K., Gatheru, Z., Tukei, P. M., Chiba, S. (1998). Prevalence of Human Calicivirus Infections in Kenya as Determined by Enzyme Immunoassays for Three Genogroups of the Virus. J. Clin. Microbiol. 36: 3160-3163 [Abstract] [Full Text]  
• Grohmann, G. S., Glass, R. I., Pereira, H. G., Monroe, S. S., Hightower, A. W., Weber, R., Bryan, R. T., The Enteric Opportunistic Infections Working Group, (1993). Enteric Viruses and Diarrhea in HIV-Infected Patients. NEJM 329: 14-20 [Abstract] [Full Text]