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J Clin Microbiol. 1985 August; 22(2): 286-291

Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3.

R D Shaw, D L Stoner-Ma, M K Estes and H B Greenberg

ABSTRACT

We prepared monoclonal antibodies against two serotypically distinct rotavirus strains: Wa, a serotype 1 virus of human origin, and rhesus rotavirus, a simian serotype 3 virus. Monoclonal antibodies which react specifically with VP7 of each serotype were identified by hemagglutination inhibition tests, plaque reduction neutralization studies, and solid-phase immunoassays which used wild-type and reassortant strains of rotavirus. An enzyme-linked immunoassay was designed which utilizes two of these antibodies to correctly identify serotype 1 and serotype 3 viruses.

J Clin Microbiol. 1985 August; 22(2): 286-291




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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 1985 by the American Society for Microbiology. All rights reserved.