This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Morris, G K Articles by Brenner, D J Search for Related Content PubMed PubMed Citation Articles by Morris, G K Articles by Brenner, D J

 Previous Article  |  Next Article 

J Clin Microbiol. 1985 November; 22(5): 714-718

Comparison of four hippurate hydrolysis methods for identification of thermophilic Campylobacter spp.

ABSTRACT

The test for hippurate hydrolysis is critical for separation of Campylobacter jejuni and C. coli strains. Glycine and benzoic acid are formed when hippurate is hydrolyzed by C. jejuni. The test used in most laboratories is one of several variations of the ninhydrin tube test described by Hwang and Ederer (M. Hwang and G. M. Ederer, J. Clin. Microbiol. 1:114-115, 1975) for detection of glycine. We evaluated three modifications of the Hwang and Ederer method and the gas-liquid chromatographic (GLC) method described by Kodaka et al. (H. Kodaka, G. L. Lombard, and V. R. Dowell, Jr., J. Clin. Microbiol. 16:962-964, 1982) for detecting benzoic acid. Campylobacter strains comprised 22 C. jejuni, 11 C. coli, and 8 C. laridis strains. The species identification of each strain was confirmed by DNA relatedness. All strains of C. jejuni were positive and all strains of C. coli and C. laridis were negative by the GLC method for detecting hippurate hydrolysis, whereas three strains of C. jejuni gave negative or variable results in the tube tests. The GLC method is more sensitive than the tube methods for detecting hippurate hydrolysis and should be used on cultures yielding variable or questionable test results.

This article has been cited by other articles:

• Korczak, B. M., Stieber, R., Emler, S., Burnens, A. P., Frey, J., Kuhnert, P. (2006). Genetic relatedness within the genus Campylobacter inferred from rpoB sequences.. Int. J. Syst. Evol. Microbiol. 56: 937-945 [Abstract] [Full Text]  
• Hill, J. E., Paccagnella, A., Law, K., Melito, P. L., Woodward, D. L., Price, L., Leung, A. H., Ng, L.-K., Hemmingsen, S. M., Goh, S. H. (2006). Identification of Campylobacter spp. and discrimination from Helicobacter and Arcobacter spp. by direct sequencing of PCR-amplified cpn60 sequences and comparison to cpnDB, a chaperonin reference sequence database.. J Med Microbiol 55: 393-399 [Abstract] [Full Text]  
• Dedieu, L., Pages, J.-M., Bolla, J.-M. (2004). Use of the omp50 Gene for Identification of Campylobacter Species by PCR. J. Clin. Microbiol. 42: 2301-2305 [Abstract] [Full Text]  
• On, S. L. W., Jordan, P. J. (2003). Evaluation of 11 PCR Assays for Species-Level Identification of Campylobacter jejuni and Campylobacter coli. J. Clin. Microbiol. 41: 330-336 [Abstract] [Full Text]