This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sompolinsky, D Articles by Malik, Z Search for Related Content PubMed PubMed Citation Articles by Sompolinsky, D Articles by Malik, Z

 Previous Article  |  Next Article 

J Clin Microbiol. 1985 November; 22(5): 828-834

Encapsulation and capsular types in isolates of Staphylococcus aureus from different sources and relationship to phage types.

ABSTRACT

The relationship of capsular types of Staphylococcus aureus to type of infection, carrier state, and phage type was studied in a collection of 477 isolates from 380 infection sites. Capsular polysaccharides were demonstrated by precipitation and agglutination with 11 monospecific antisera. When only one isolate from each infection was considered, 63% were of type 8 and 16% were of type 5. Of all the isolates tested, over 90% were encapsulated. We did not demonstrate any marked difference in the distribution of capsular types between isolates from the blood stream or purulent processes and isolates from healthy carriers or food. Most isolates from bovine mastitis milk had nontypeable capsules. The capsular type seemed stable in culture, and encapsulation had no apparent influence on susceptibility to phages. Of 27 phage-propagating strains maintained via culture transfer on artificial media over many years, 16 (59%) produced capsules. A striking association between certain phage patterns and capsular types was demonstrated.

This article has been cited by other articles:

• Tuchscherr, L. P. N., Gomez, M. I., Buzzola, F. R., Calvinho, L. F., Lee, J. C., Sordelli, D. O. (2007). Characterization of a New Variant of IS257 That Has Displaced the Capsule Genes within Bovine Isolates of Staphylococcus aureus. Infect. Immun. 75: 5483-5488 [Abstract] [Full Text]  
• Verdier, I., Durand, G., Bes, M., Taylor, K. L., Lina, G., Vandenesch, F., Fattom, A. I., Etienne, J. (2007). Identification of the Capsular Polysaccharides in Staphylococcus aureus Clinical Isolates by PCR and Agglutination Tests. J. Clin. Microbiol. 45: 725-729 [Abstract] [Full Text]  
• Tuchscherr, L. P. N., Buzzola, F. R., Alvarez, L. P., Caccuri, R. L., Lee, J. C., Sordelli, D. O. (2005). Capsule-Negative Staphylococcus aureus Induces Chronic Experimental Mastitis in Mice. Infect. Immun. 73: 7932-7937 [Abstract] [Full Text]  
• Rich, J., Lee, J. C. (2005). The Pathogenesis of Staphylococcus aureus Infection in the Diabetic NOD Mouse. Diabetes 54: 2904-2910 [Abstract] [Full Text]  
• Watts, A., Ke, D., Wang, Q., Pillay, A., Nicholson-Weller, A., Lee, J. C. (2005). Staphylococcus aureus Strains That Express Serotype 5 or Serotype 8 Capsular Polysaccharides Differ in Virulence. Infect. Immun. 73: 3502-3511 [Abstract] [Full Text]  
• Mulrooney, E. F., Poon, K. K. H., McNally, D. J., Brisson, J.-R., Lam, J. S. (2005). Biosynthesis of UDP-N-acetyl-L-fucosamine, a Precursor to the Biosynthesis of Lipopolysaccharide in Pseudomonas aeruginosa Serotype O11. J. Biol. Chem. 280: 19535-19542 [Abstract] [Full Text]  
• Kampen, A. H., Tollersrud, T., Lund, A. (2005). Staphylococcus aureus Capsular Polysaccharide Types 5 and 8 Reduce Killing by Bovine Neutrophils In Vitro. Infect. Immun. 73: 1578-1583 [Abstract] [Full Text]  
• Lee, L. Y. L., Liang, X., Hook, M., Brown, E. L. (2004). Identification and Characterization of the C3 Binding Domain of the Staphylococcus aureus Extracellular Fibrinogen-binding Protein (Efb). J. Biol. Chem. 279: 50710-50716 [Abstract] [Full Text]  
• Cunnion, K. M., Hair, P. S., Buescher, E. S. (2004). Cleavage of Complement C3b to iC3b on the Surface of Staphylococcus aureus Is Mediated by Serum Complement Factor I. Infect. Immun. 72: 2858-2863 [Abstract] [Full Text]  
• O'Riordan, K., Lee, J. C. (2004). Staphylococcus aureus Capsular Polysaccharides. Clin. Microbiol. Rev. 17: 218-234 [Abstract] [Full Text]  
• Cunnion, K. M., Frank, M. M. (2003). Complement Activation Influences Staphylococcus aureus Adherence to Endothelial Cells. Infect. Immun. 71: 1321-1327 [Abstract] [Full Text]  
• Cunnion, K. M., Zhang, H.-M., Frank, M. M. (2003). Availability of Complement Bound to Staphylococcus aureus To Interact with Membrane Complement Receptors Influences Efficiency of Phagocytosis. Infect. Immun. 71: 656-662 [Abstract] [Full Text]  
• Kneidinger, B., O'Riordan, K., Li, J., Brisson, J.-R., Lee, J. C., Lam, J. S. (2003). Three Highly Conserved Proteins Catalyze the Conversion of UDP-N-acetyl-D-glucosamine to Precursors for the Biosynthesis of O Antigen in Pseudomonas aeruginosa O11 and Capsule in Staphylococcus aureus Type 5. IMPLICATIONS FOR THE UDP-N-ACETYL-L-FUCOSAMINE BIOSYNTHETIC PATHWAY. J. Biol. Chem. 278: 3615-3627 [Abstract] [Full Text]  
• Herron, L. L., Chakravarty, R., Dwan, C., Fitzgerald, J. R., Musser, J. M., Retzel, E., Kapur, V. (2002). Genome Sequence Survey Identifies Unique Sequences and Key Virulence Genes with Unusual Rates of Amino Acid Substitution in Bovine Staphylococcus aureus. Infect. Immun. 70: 3978-3981 [Abstract] [Full Text]  
• Cunnion, K. M., Lee, J. C., Frank, M. M. (2001). Capsule Production and Growth Phase Influence Binding of Complement to Staphylococcus aureus. Infect. Immun. 69: 6796-6803 [Abstract] [Full Text]  
• Herbert, S., Newell, S. W., Lee, C., Wieland, K.-P., Dassy, B., Fournier, J.-M., Wolz, C., Doring, G. (2001). Regulation of Staphylococcus aureus Type 5 and Type 8 Capsular Polysaccharides by CO2. J. Bacteriol. 183: 4609-4613 [Abstract] [Full Text]  
• Cramton, S. E., Ulrich, M., Gotz, F., Doring, G. (2001). Anaerobic Conditions Induce Expression of Polysaccharide Intercellular Adhesin in Staphylococcus aureus and Staphylococcus epidermidis. Infect. Immun. 69: 4079-4085 [Abstract] [Full Text]  
• Blake, J. E., Metcalfe, M. A. (2001). A Shared Noncapsular Antigen Is Responsible for False-Positive Reactions by Staphylococcus epidermidis in Commercial Agglutination Tests for Staphylococcus aureus. J. Clin. Microbiol. 39: 544-550 [Abstract] [Full Text]  
• Pohlmann-Dietze, P., Ulrich, M., Kiser, K. B., Doring, G., Lee, J. C., Fournier, J.-M., Botzenhart, K., Wolz, C. (2000). Adherence of Staphylococcus aureus to Endothelial Cells: Influence of Capsular Polysaccharide, Global Regulator agr, and Bacterial Growth Phase. Infect. Immun. 68: 4865-4871 [Abstract] [Full Text]  
• Tollersrud, T., Kenny, K., Reitz, A. J. Jr., Lee, J. C. (2000). Genetic and Serologic Evaluation of Capsule Production by Bovine Mammary Isolates of Staphylococcus aureus and Other Staphylococcus spp. from Europe and the United States. J. Clin. Microbiol. 38: 2998-3003 [Abstract] [Full Text]  
• Nair, S. P., Williams, R. J., Henderson, B. (2000). Advances in our understanding of the bone and joint pathology caused by Staphylococcus aureus infection. Rheumatology (Oxford) 39: 821-834 [Full Text]  
• Boyle-Vavra, S., Berke, S. K., Lee, J. C., Daum, R. S. (2000). Reversion of the Glycopeptide Resistance Phenotype in Staphylococcus aureus Clinical Isolates. Antimicrob. Agents Chemother. 44: 272-277 [Abstract] [Full Text]  
• Sordelli, D. O., Buzzola, F. R., Gomez, M. I., Steele-Moore, L., Berg, D., Gentilini, E., Catalano, M., Reitz, A. J., Tollersrud, T., Denamiel, G., Jeric, P., Lee, J. C. (2000). Capsule Expression by Bovine Isolates of Staphylococcus aureus from Argentina: Genetic and Epidemiologic Analyses. J. Clin. Microbiol. 38: 846-850 [Abstract] [Full Text]  
• Snodgrass, J. L., Mohamed, N., Ross, J. M., Sau, S., Lee, C. Y., Smeltzer, M. S. (1999). Functional Analysis of the Staphylococcus aureus Collagen Adhesin B Domain. Infect. Immun. 67: 3952-3959 [Abstract] [Full Text]  
• Ouyang, S., Sau, S., Lee, C. Y. (1999). Promoter Analysis of the cap8 Operon, Involved in Type 8 Capsular Polysaccharide Production in Staphylococcus aureus. J. Bacteriol. 181: 2492-2500 [Abstract] [Full Text]  
• Thakker, M., Park, J.-S., Carey, V., Lee, J. C. (1998). Staphylococcus aureus Serotype 5 Capsular Polysaccharide Is Antiphagocytic and Enhances Bacterial Virulence in a Murine Bacteremia Model. Infect. Immun. 66: 5183-5189 [Abstract] [Full Text]  
• Fattom, A. I., Sarwar, J., Basham, L., Ennifar, S., Naso, R. (1998). Antigenic Determinants of Staphylococcus aureus Type 5 and Type 8 Capsular Polysaccharide Vaccines. Infect. Immun. 66: 4588-4592 [Abstract] [Full Text]  
• Fox, K. F., Stewart, G. C., Fox, A. (1998). Synthesis of Microcapsule by Staphylococcus aureus Is Not Responsive to Environmental Phosphate Concentrations. Infect. Immun. 66: 4004-4007 [Abstract] [Full Text]  
• Na'was, T., Hawwari, A., Hendrix, E., Hebden, J., Edelman, R., Martin, M., Campbell, W., Naso, R., Schwalbe, R., Fattom, A. I. (1998). Phenotypic and Genotypic Characterization of Nosocomial Staphylococcus aureus Isolates from Trauma Patients. J. Clin. Microbiol. 36: 414-420 [Abstract] [Full Text]