This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Edinger, R C Articles by Nolte, F S Search for Related Content PubMed PubMed Citation Articles by Edinger, R C Articles by Nolte, F S

 Previous Article  |  Next Article 

J Clin Microbiol. 1985 December; 22(6): 1063-1065

Supplementary rapid biochemical test panel for the API 20E bacterial identification system.

ABSTRACT

The API 20E Analytical Profile Index typically suggests three or four conventional biochemical tests to complete the identification of strains either identified to genus only or that have multiple genera consistent with the profile number. We compiled a simple panel of eight rapid (4-h) tests that can substitute for the supplementary biochemical tests recommended by Analytab Products (Plainview, N.Y.). The rapid test panel (RTP) consisted of adonitol, cellobiose, lactose, raffinose, rhamnose, and xylose utilization, lysine decarboxylase activity, and motility. A total of 114 consecutive clinical isolates that required additional tests to complete the identifications were each tested with the complete RTP, as well as with the recommended conventional biochemicals. All discordant identifications were resolved by using an expanded series of conventional biochemical tests. Overall, 110 (96%) strains were identified to the correct genus, and 109 (95%) strains were identified to the correct species by using the RTP, as compared with 105 (92%) identified to the correct genus and 90 (79%) identified to the correct species with the recommended tests. The identifications based on the two supplementary test systems did not agree for 7 (6.1%) strains. Four discrepancies were resolved in favor of the RTP, and three were resolved in favor of the recommended tests. We were unable to identify five (4.4%) strains with the recommended tests and only one (0.9%) with the RTP. A majority (86%) of the test strains were identified to the species level with the RTP after only 4 h of incubation.