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J Clin Microbiol. 1986 April; 23(4): 800-802

Enhanced isolation of respiratory syncytial virus in cell culture.

M Q Arens, E M Swierkosz, R R Schmidt, T Armstrong and K A Rivetna

ABSTRACT

During two winter seasons, we found that the combination of WI-38 or MRC-5 human lung fibroblasts plus primary rhesus monkey kidney (RhMK) and HEp-2 cell cultures yielded maximal isolation of respiratory syncytial virus. Cytopathic effects (CPE) developed earliest in RhMK cells and slowest in the human fibroblast lines. In RhMK cells, 50% of ultimately positive cultures showed CPE in 5 days, and 90% of positive cultures showed CPE within 7 days during both respiratory syncytial virus seasons.

J Clin Microbiol. 1986 April; 23(4): 800-802






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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 1986 by the American Society for Microbiology. All rights reserved.