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J Clin Microbiol. 1986 July; 24(1): 149-151

Evaluation and optimization of the DNA filter assay for direct detection of enterotoxigenic Escherichia coli in the presence of stool coliforms.

W C Yam, M L Lung and M H Ng

ABSTRACT

Direct detection of enterotoxigenic Escherichia coli (ETEC) in mixed cultures by DNA filter assay was affected by the presence of other bacteria in the cultures. By shortening incubation to 6 h, bacterial overgrowth was minimized, allowing direct detection of ETEC in stools or mixed cultures initially containing 0.2 to 3% ETEC.

J Clin Microbiol. 1986 July; 24(1): 149-151






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