This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pedersen, S S Articles by Høiby, N Search for Related Content PubMed PubMed Citation Articles by Pedersen, S S Articles by Høiby, N

 Previous Article  |  Next Article 

J Clin Microbiol. 1987 October; 25(10): 1830-1836

Diagnosis of chronic Pseudomonas aeruginosa infection in cystic fibrosis by enzyme-linked immunosorbent assay.

Department of Clinical Microbiology, Statens Seruminstitut, Copenhagen, Denmark.

ABSTRACT

An easily applicable test for diagnosis of chronic Pseudomonas aeruginosa infection in cystic fibrosis by enzyme-linked immunosorbent assay (ELISA) for determination of serum immunoglobulin G to P. aeruginosa was developed. Soluble antigens obtained by ultrasonication of P. aeruginosa, serotypes O:1 to O:17, were used as antigens immobilized to polystyrene microtiter plates. The intraplate, plate-to-plate, and day-to-day variations were 14, 19, and 20%, respectively. Plates coated with the antigens could be stored for at least 64 days at +4 and +22 degrees C without any significant change in activity. Normal values were determined in sera from 164 controls (100 children and 64 adults). The sensitivity and specificity of the ELISA was determined by using serum samples from 243 cystic fibrosis patients and were compared to results with crossed immunoelectrophoresis (CIE). The ELISA could diagnose chronic P. aeruginosa infection with a diagnostic sensitivity of 93% and specificity of 92%. The sensitivity and specificity for the diagnosis of the early stages of chronic P. aeruginosa infection by a single sample were 90 and 100%, respectively, and by using an increased antibody response in paired samples, the sensitivity was 93% and specificity was 87%. There was a statistically significant correlation between antibody levels obtained by ELISA and those obtained by CIE. The sensitivity and specificity of the ELISA were equal to those of CIE, and because of its simplicity, the ELISA is recommended as a routine test in patients with cystic fibrosis.

This article has been cited by other articles:

• Tramper-Stranders, G A, van der Ent, C K, Slieker, M G, Terheggen-Lagro, S W J, Teding van Berkhout, F, Kimpen, J L L, Wolfs, T F W (2006). Diagnostic value of serological tests against Pseudomonas aeruginosa in a large cystic fibrosis population. Thorax 61: 689-693 [Abstract] [Full Text]  
• Caballero, E, Drobnic, M-E, Perez, M-T, Manresa, J-M, Ferrer, A, Orriols, R (2001). Anti-Pseudomonas aeruginosa antibody detection in patients with bronchiectasis without cystic fibrosis. Thorax 56: 669-674 [Abstract] [Full Text]  
• Skov, M, Pressler, T, Jensen, H E, Høiby, N, Koch, C (1999). Specific IgG subclass antibody pattern to Aspergillus fumigatus in patients with cystic fibrosis with allergic bronchopulmonary aspergillosis (ABPA). Thorax 54: 44-50 [Abstract] [Full Text]  
• BISGAARD, H., PEDERSEN, S. S., NIELSEN, K. G., SKOV, M., LAURSEN, E. M., KRONBORG, G., REIMERT, C. M., OIBY, N. H, KOCH, C. (1997). Controlled Trial of Inhaled Budesonide in Patients with Cystic Fibrosis and Chronic Bronchopulmonary Pseudomonas aeruginosa Infection. Am. J. Respir. Crit. Care Med. 156: 1190-1196 [Abstract] [Full Text]  
• Pier, G., Small, G., Warren, H. (1990). Protection against mucoid Pseudomonas aeruginosa in rodent models of endobronchial infections. Science 249: 537-540 [Abstract]