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J Clin Microbiol. 1987 November; 25(11): 2102-2104

Efficacy of enzyme-linked immunosorbent assay for rapid diagnosis of Bordetella pertussis infection.

Department of Microbiology, Adelaide Children's Hospital, South Australia.

ABSTRACT

We examined the diagnostic efficacy of an enzyme-linked immunosorbent assay (ELISA) for class-specific antibodies to Bordetella pertussis in acute-phase sera collected from 1,240 patients with suspected pertussis. A total of 833 serum specimens (67%) yielded positive results. The proportion of positive results increased to 77% if a second (convalescent-phase) serum was also tested. By comparison, a bacterial agglutination test for B. pertussis antibodies was positive in only 21% of acute-phase specimens and 50% of paired specimens. The high proportion of acute-phase sera which were ELISA positive indicates that a measurable serologic response has usually occurred by the time the diagnosis is suspected. Thus, the ELISA is potentially the most rapid means of laboratory confirmation of B. pertussis infection.

This article has been cited by other articles:

• Kosters, K., Riffelmann, M., Dohrn, B., von Konig, C. H. W. (2000). Comparison of Five Commercial Enzyme-Linked Immunosorbent Assays for Detection of Antibodies to Bordetella pertussis. CVI 7: 422-426 [Abstract] [Full Text]