This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Scherer, S Articles by Stevens, D A Search for Related Content PubMed PubMed Citation Articles by Scherer, S Articles by Stevens, D A

 Previous Article  |  Next Article 

J Clin Microbiol. 1987 April; 25(4): 675-679

Application of DNA typing methods to epidemiology and taxonomy of Candida species.

ABSTRACT

Methods are described for extraction of DNA from the yeast form of Candida spp., followed by digestion and electrophoresis of DNA fragments. The resulting gel patterns (greater than 100 bands) were used to type Candida isolates. Four intense bands identified, three of which are present in each isolate (6 to 7, 3.7 or 4.2, and 2.5 to 3 kilobases), appear to be DNA encoding the rRNA. The methods proved to be both simple and reproducible. The patterns were shown to be stable through several hundred doublings from multiple single colonies. A survey of isolates showed that, on the basis of similarity of gel patterns, several Candida species could be sorted into mutually exclusive groups, and subgroups could be created. Analyses of this survey suggested the possible epidemiologic and taxonomic applications of these methods. DNA typing methods appear to offer important potential advantages over phenotyping methods. The methods provide a base for further epidemiologic studies and for further development of techniques, such as the use of cloned probes for studies of DNA homology.

This article has been cited by other articles:

• Chapeland-Leclerc, F., Paccallet, P., Ruprich-Robert, G., Reboutier, D., Chastin, C., Papon, N. (2007). Differential Involvement of Histidine Kinase Receptors in Pseudohyphal Development, Stress Adaptation, and Drug Sensitivity of the Opportunistic Yeast Candida lusitaniae. Eukaryot Cell 6: 1782-1794 [Abstract] [Full Text]  
• Liguori, G., Lucariello, A., Colella, G., De Luca, A., Marinelli, P. (2007). Rapid identification of Candida species in oral rinse solutions by PCR. J. Clin. Pathol. 60: 1035-1039 [Abstract] [Full Text]  
• Girmenia, C., Pizzarelli, G., Cristini, F., Barchiesi, F., Spreghini, E., Scalise, G., Martino, P. (2006). Candida guilliermondii Fungemia in Patients with Hematologic Malignancies.. J. Clin. Microbiol. 44: 2458-2464 [Abstract] [Full Text]  
• Chapeland-Leclerc, F., Bouchoux, J., Goumar, A., Chastin, C., Villard, J., Noel, T. (2005). Inactivation of the FCY2 Gene Encoding Purine-Cytosine Permease Promotes Cross-Resistance to Flucytosine and Fluconazole in Candida lusitaniae. Antimicrob. Agents Chemother. 49: 3101-3108 [Abstract] [Full Text]  
• Sarvikivi, E., Lyytikainen, O., Soll, D. R., Pujol, C., Pfaller, M. A., Richardson, M., Koukila-Kahkola, P., Luukkainen, P., Saxen, H. (2005). Emergence of Fluconazole Resistance in a Candida parapsilosis Strain That Caused Infections in a Neonatal Intensive Care Unit. J. Clin. Microbiol. 43: 2729-2735 [Abstract] [Full Text]  
• Barchiesi, F, Tortorano, A M, Di Francesco, L F., Rigoni, A, Giacometti, A, Spreghini, E, Scalise, G, Viviani, M A (2005). Genotypic variation and antifungal susceptibilities of Candida pelliculosa clinical isolates. J Med Microbiol 54: 279-285 [Abstract] [Full Text]  
• Tavanti, A., Davidson, A. D., Gow, N. A. R., Maiden, M. C. J., Odds, F. C. (2005). Candida orthopsilosis and Candida metapsilosis spp. nov. To Replace Candida parapsilosis Groups II and III. J. Clin. Microbiol. 43: 284-292 [Abstract] [Full Text]  
• Sancak, B., Rex, J. H., Chen, E., Marr, K. (2004). Comparison of PCR- and HinfI Restriction Endonuclease-Based Methods for Typing of Candida krusei Isolates. J. Clin. Microbiol. 42: 5889-5891 [Abstract] [Full Text]  
• Stevens, D. A., Espiritu, M., Parmar, R. (2004). Paradoxical Effect of Caspofungin: Reduced Activity against Candida albicans at High Drug Concentrations. Antimicrob. Agents Chemother. 48: 3407-3411 [Abstract] [Full Text]  
• Shin, J. H., Park, M.-R., Song, J. W., Shin, D. H., Jung, S.-I., Cho, D., Kee, S. J., Shin, M. G., Suh, S. P., Ryang, D. W. (2004). Microevolution of Candida albicans Strains during Catheter-Related Candidemia. J. Clin. Microbiol. 42: 4025-4031 [Abstract] [Full Text]  
• Dodgson, A. R., Dodgson, K. J., Pujol, C., Pfaller, M. A., Soll, D. R. (2004). Clade-Specific Flucytosine Resistance Is Due to a Single Nucleotide Change in the FUR1 Gene of Candida albicans. Antimicrob. Agents Chemother. 48: 2223-2227 [Abstract] [Full Text]  
• Shukla, S., Saini, P., Smriti, , Jha, S., Ambudkar, S. V., Prasad, R. (2003). Functional Characterization of Candida albicans ABC Transporter Cdr1p. Eukaryot Cell 2: 1361-1375 [Abstract] [Full Text]  
• Zhang, N., Harrex, A. L., Holland, B. R., Fenton, L. E., Cannon, R. D., Schmid, J. (2003). Sixty Alleles of the ALS7 Open Reading Frame in Candida albicans: ALS7 Is a Hypermutable Contingency Locus. Genome Res 13: 2005-2017 [Abstract] [Full Text]  
• Himmelreich, U., Somorjai, R. L., Dolenko, B., Lee, O. C., Daniel, H.-M., Murray, R., Mountford, C. E., Sorrell, T. C. (2003). Rapid Identification of Candida Species by Using Nuclear Magnetic Resonance Spectroscopy and a Statistical Classification Strategy. Appl. Environ. Microbiol. 69: 4566-4574 [Abstract] [Full Text]  
• Blignaut, E., Pujol, C., Joly, S., Soll, D. R. (2003). Racial Distribution of Candida dubliniensis Colonization among South Africans. J. Clin. Microbiol. 41: 1838-1842 [Abstract] [Full Text]  
• Pujol, C., Messer, S. A., Pfaller, M., Soll, D. R. (2003). Drug Resistance Is Not Directly Affected by Mating Type Locus Zygosity in Candida albicans. Antimicrob. Agents Chemother. 47: 1207-1212 [Abstract] [Full Text]  
• Sampaio, P., Gusmao, L., Alves, C., Pina-Vaz, C., Amorim, A., Pais, C. (2003). Highly Polymorphic Microsatellite for Identification of Candida albicans Strains. J. Clin. Microbiol. 41: 552-557 [Abstract] [Full Text]  
• Lockhart, S. R., Daniels, K. J., Zhao, R., Wessels, D., Soll, D. R. (2003). Cell Biology of Mating in Candida albicans. Eukaryot Cell 2: 49-61 [Abstract] [Full Text]  
• Sancak, B., Rex, J. H., Paetznick, V., Chen, E., Rodriguez, J. (2003). Evaluation of a Method for Identification of Candida dubliniensis Bloodstream Isolates. J. Clin. Microbiol. 41: 489-491 [Abstract] [Full Text]  
• Wada, S.-i., Niimi, M., Niimi, K., Holmes, A. R., Monk, B. C., Cannon, R. D., Uehara, Y. (2002). Candida glabrata ATP-binding Cassette Transporters Cdr1p and Pdh1p Expressed in a Saccharomyces cerevisiae Strain Deficient in Membrane Transporters Show Phosphorylation-dependent Pumping Properties. J. Biol. Chem. 277: 46809-46821 [Abstract] [Full Text]  
• Lockhart, S. R., Pujol, C., Daniels, K. J., Miller, M. G., Johnson, A. D., Pfaller, M. A., Soll, D. R. (2002). In Candida albicans, White-Opaque Switchers Are Homozygous for Mating Type. Genetics 162: 737-745 [Abstract] [Full Text]  
• Pujol, C., Pfaller, M., Soll, D. R. (2002). Ca3 Fingerprinting of Candida albicans Bloodstream Isolates from the United States, Canada, South America, and Europe Reveals a European Clade. J. Clin. Microbiol. 40: 2729-2740 [Abstract] [Full Text]  
• Stevens, D. A. (2002). Molecular Epidemiology of Candida. J. Clin. Microbiol. 40: 2710-2710 [Full Text]  
• Zhao, R., Lockhart, S. R., Daniels, K., Soll, D. R. (2002). Roles of TUP1 in Switching, Phase Maintenance, and Phase-Specific Gene Expression in Candida albicans. Eukaryot Cell 1: 353-365 [Abstract] [Full Text]  
• Nosek, J., Tomaska, L'u., Rycovska, A., Fukuhara, H. (2002). Mitochondrial Telomeres as Molecular Markers for Identification of the Opportunistic Yeast Pathogen Candida parapsilosis. J. Clin. Microbiol. 40: 1283-1289 [Abstract] [Full Text]  
• Blignaut, E., Pujol, C., Lockhart, S., Joly, S., Soll, D. R. (2002). Ca3 Fingerprinting of Candida albicans Isolates from Human Immunodeficiency Virus-Positive and Healthy Individuals Reveals a New Clade in South Africa. J. Clin. Microbiol. 40: 826-836 [Abstract] [Full Text]  
• Vargas, K. G., Joly, S. (2002). Carriage Frequency, Intensity of Carriage, and Strains of Oral Yeast Species Vary in the Progression to Oral Candidiasis in Human Immunodeficiency Virus-Positive Individuals. J. Clin. Microbiol. 40: 341-350 [Abstract] [Full Text]  
• Gee, S. F., Joly, S., Soll, D. R., Meis, J. F. G. M., Verweij, P. E., Polacheck, I., Sullivan, D. J., Coleman, D. C. (2002). Identification of Four Distinct Genotypes of Candida dubliniensis and Detection of Microevolution In Vitro and In Vivo. J. Clin. Microbiol. 40: 556-574 [Abstract] [Full Text]  
• Nakamura, K., Niimi, M., Niimi, K., Holmes, A. R., Yates, J. E., Decottignies, A., Monk, B. C., Goffeau, A., Cannon, R. D. (2001). Functional Expression of Candida albicans Drug Efflux Pump Cdr1p in a Saccharomyces cerevisiae Strain Deficient in Membrane Transporters. Antimicrob. Agents Chemother. 45: 3366-3374 [Abstract] [Full Text]  
• Bartie, K. L., Williams, D. W., Wilson, M. J., Potts, A. J. C., Lewis, M. A. O. (2001). PCR Fingerprinting of Candida albicans Associated with Chronic Hyperplastic Candidosis and Other Oral Conditions. J. Clin. Microbiol. 39: 4066-4075 [Abstract] [Full Text]  
• Niimi, K., Shepherd, M. G., Cannon, R. D. (2001). Distinguishing Candida Species by {beta}-N-Acetylhexosaminidase Activity. J. Clin. Microbiol. 39: 2089-2097 [Abstract] [Full Text]  
• El-Din, S S, Reynolds, M T, Ashbee, H R, Barton, R C, Evans, E G V (2001). An investigation into the pathogenesis of vulvo-vaginal candidosis. Sex. Transm. Infect. 77: 179-183 [Abstract] [Full Text]  
• Bahn, Y.-S., Sundstrom, P. (2001). CAP1, an Adenylate Cyclase-Associated Protein Gene, Regulates Bud-Hypha Transitions, Filamentous Growth, and Cyclic AMP Levels and Is Required for Virulence of Candida albicans. J. Bacteriol. 183: 3211-3223 [Abstract] [Full Text]  
• PONTIERI, E., CARACCIOLO, C., BIANCHINI, S., D'ANTONIO, D., NOVELLI, G., DALLAPICCOLA, B., CARRUBA, G. (2001). Single primer pair for PCR identification of Candida parapsilosis group I isolates. J Med Microbiol 50: 441-448 [Abstract] [Full Text]  
• Enger, L., Joly, S., Pujol, C., Simonson, P., Pfaller, M., Soll, D. R. (2001). Cloning and Characterization of a Complex DNA Fingerprinting Probe for Candida parapsilosis. J. Clin. Microbiol. 39: 658-669 [Abstract] [Full Text]  
• Vargas, K., Messer, S. A., Pfaller, M., Lockhart, S. R., Stapleton, J. T., Hellstein, J., Soll, D. R. (2000). Elevated Phenotypic Switching and Drug Resistance of Candida albicans from Human Immunodeficiency Virus-Positive Individuals prior to First Thrush Episode. J. Clin. Microbiol. 38: 3595-3607 [Abstract] [Full Text]  
• Soll, D. R. (2000). The Ins and Outs of DNA Fingerprinting the Infectious Fungi. Clin. Microbiol. Rev. 13: 332-370 [Abstract] [Full Text]  
• Cowen, L. E., Sanglard, D., Calabrese, D., Sirjusingh, C., Anderson, J. B., Kohn, L. M. (2000). Evolution of Drug Resistance in Experimental Populations of Candida albicans. J. Bacteriol. 182: 1515-1522 [Abstract] [Full Text]  
• Lachke, S. A., Srikantha, T., Tsai, L. K., Daniels, K., Soll, D. R. (2000). Phenotypic Switching in Candida glabrata Involves Phase-Specific Regulation of the Metallothionein Gene MT-II and the Newly Discovered Hemolysin Gene HLP. Infect. Immun. 68: 884-895 [Abstract] [Full Text]  
• Cowen, L. E., Sirjusingh, C., Summerbell, R. C., Walmsley, S., Richardson, S., Kohn, L. M., Anderson, J. B. (1999). Multilocus Genotypes and DNA Fingerprints Do Not Predict Variation in Azole Resistance among Clinical Isolates of Candida albicans. Antimicrob. Agents Chemother. 43: 2930-2938 [Abstract] [Full Text]  
• Posteraro, B., Sanguinetti, M., D'Amore, G., Masucci, L., Morace, G., Fadda, G. (1999). Molecular and Epidemiological Characterization of Vaginal Saccharomyces cerevisiae Isolates. J. Clin. Microbiol. 37: 2230-2235 [Abstract] [Full Text]  
• McNeil, M. M., Lasker, B. A., Lott, T. J., Jarvis, W. R. (1999). Postsurgical Candida albicans Infections Associated with an Extrinsically Contaminated Intravenous Anesthetic Agent. J. Clin. Microbiol. 37: 1398-1403 [Abstract] [Full Text]  
• Joly, S., Pujol, C., Rysz, M., Vargas, K., Soll, D. R. (1999). Development and Characterization of Complex DNA Fingerprinting Probes for the Infectious Yeast Candida dubliniensis. J. Clin. Microbiol. 37: 1035-1044 [Abstract] [Full Text]  
• McCullough, M. J., Clemons, K. V., Stevens, D. A. (1999). Molecular and Phenotypic Characterization of Genotypic Candida albicans Subgroups and Comparison with Candida dubliniensis and Candida stellatoidea. J. Clin. Microbiol. 37: 417-421 [Abstract] [Full Text]  
• Lockhart, S. R., Nguyen, M., Srikantha, T., Soll, D. R. (1998). A MADS Box Protein Consensus Binding Site Is Necessary and Sufficient for Activation of the Opaque-Phase-Specific Gene OP4 of Candida albicans. J. Bacteriol. 180: 6607-6616 [Abstract] [Full Text]  
• McCullough, M. J., Clemons, K. V., McCusker, J. H., Stevens, D. A. (1998). Species Identification and Virulence Attributes of Saccharomyces boulardii (nom. inval.). J. Clin. Microbiol. 36: 2613-2617 [Abstract] [Full Text]  
• Pfaller, M. A., Lockhart, S. R., Pujol, C., Swails-Wenger, J. A., Messer, S. A., Edmond, M. B., Jones, R. N., Wenzel, R. P., Soll, D. R. (1998). Hospital Specificity, Region Specificity, and Fluconazole Resistance of Candida albicans Bloodstream Isolates. J. Clin. Microbiol. 36: 1518-1529 [Abstract] [Full Text]  
• McCullough, M. J., Clemons, K. V., McCusker, J. H., Stevens, D. A. (1998). Intergenic Transcribed Spacer PCR Ribotyping for Differentiation of Saccharomyces Species and Interspecific Hybrids. J. Clin. Microbiol. 36: 1035-1038 [Abstract] [Full Text]  
• Chang, H. J., Miller, H. L., Watkins, N., Arduino, M. J., Ashford, D. A., Midgley, G., Aguero, S. M., Pinto-Powell, R., von Reyn, C. F., Edwards, W., McNeil, M. M., Jarvis, W. R., Pruitt, R. (1998). An Epidemic of Malassezia pachydermatis in an Intensive Care Nursery Associated with Colonization of Health Care Workers' Pet Dogs. NEJM 338: 706-711 [Abstract] [Full Text]  
• D'Antonio, D., Violante, B., Mazzoni, A., Bonfini, T., Capuani, M. A., D'Aloia, F., Iacone, A., Schioppa, F., Romano, F. (1998). A Nosocomial Cluster of Candida inconspicua Infections in Patients with Hematological Malignancies. J. Clin. Microbiol. 36: 792-795 [Abstract] [Full Text]  
• Vazquez, J. A., Dembry, L. M., Sanchez, V., Vazquez, M. A., Sobel, J. D., Dmuchowski, C., Zervos, M. J. (1998). Nosocomial Candida glabrata Colonization: an Epidemiologic Study. J. Clin. Microbiol. 36: 421-426 [Abstract] [Full Text]  
• McCullough, M. J., Clemons, K. V., Farina, C., McCusker, J. H., Stevens, D. A. (1998). Epidemiological Investigation of Vaginal Saccharomyces cerevisiae Isolates by a Genotypic Method. J. Clin. Microbiol. 36: 557-562 [Abstract] [Full Text]  
• Roy, B, Meyer, S. (1998). Confirmation of the distinct genotype groups within the form species Candida parapsilosis [In Process Citation]. J. Clin. Microbiol. 36: 216-218 [Abstract] [Full Text]