This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Péter, O Articles by Burgdorfer, W Search for Related Content PubMed PubMed Citation Articles by Péter, O Articles by Burgdorfer, W

 Previous Article  |  Next Article 

J Clin Microbiol. 1987 June; 25(6): 1063-1067

Comparison of enzyme-linked immunosorbent assay and complement fixation and indirect fluorescent-antibody tests for detection of Coxiella burnetii antibody.

ABSTRACT

An enzyme-linked immunosorbent assay (ELISA) was developed to detect immunoglobulin G to Coxiella burnetii phase II. Serum samples from 213 patients who had had Q fever 1 year previously and from 301 blood donors from six localities in Switzerland were tested by ELISA and by indirect fluorescent-antibody (IFA) and complement fixation (CF) tests. The ELISA and the IFA and CF tests detected antibody to C. burnetii in 202 (94.8%), 193 (90.6%), and 166 (77.8%) of the 213 Q fever patients, respectively. With the serum samples from blood donors, the ELISA yielded a higher percentage of positive sera than did the IFA and CF tests. The high specificity of the three tests was confirmed by analyzing paired serum samples from 36 patients suffering from acute pneumonia of viral or bacterial origin. In these cases, the serological results were negative by the three tests, except for three Q-fever cases included as positive control.

This article has been cited by other articles:

• Zhang, G., Kiss, K., Seshadri, R., Hendrix, L. R., Samuel, J. E. (2004). Identification and Cloning of Immunodominant Antigens of Coxiella burnetii. Infect. Immun. 72: 844-852 [Abstract] [Full Text]  
• Field, P. R., Santiago, A., Chan, S.-W., Patel, D. B., Dickeson, D., Mitchell, J. L., Devine, P. L., Murphy, A. M. (2002). Evaluation of a Novel Commercial Enzyme-Linked Immunosorbent Assay Detecting Coxiella burnetii-Specific Immunoglobulin G for Q Fever Prevaccination Screening and Diagnosis. J. Clin. Microbiol. 40: 3526-3529 [Abstract] [Full Text]  
• Field, P. R., Mitchell, J. L., Santiago, A., Dickeson, D. J., Chan, S.-W., Ho, D. W. T., Murphy, A. M., Cuzzubbo, A. J., Devine, P. L. (2000). Comparison of a Commercial Enzyme-Linked Immunosorbent Assay with Immunofluorescence and Complement Fixation Tests for Detection of Coxiella burnetii (Q Fever) Immunoglobulin M. J. Clin. Microbiol. 38: 1645-1647 [Abstract] [Full Text]  
• Maurin, M., Raoult, D. (1999). Q Fever. Clin. Microbiol. Rev. 12: 518-553 [Abstract] [Full Text]  
• Fournier, P.-E., Marrie, T. J., Raoult, D. (1998). Diagnosis of Q Fever. J. Clin. Microbiol. 36: 1823-1834 [Full Text]  
• Zhang, G., Nguyen, S., To, H, Ogawa, M, Hotta, A, Yamaguchi, T, Kim, H., Fukushi, H, Hirai, K (1998). Clinical evaluation of a new PCR assay for detection of Coxiella burnetii in human serum samples [In Process Citation]. J. Clin. Microbiol. 36: 77-80 [Abstract] [Full Text]