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J Clin Microbiol. 1988 January; 26(1): 158-160

Polyacrylamide gel electrophoresis and silver staining for detection of rotavirus in stools from diarrheic patients in Thailand.

S Kasempimolporn, S Louisirirotchanakul, P Sinarachatanant and C Wasi

Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.

ABSTRACT

Detection of rotavirus by polyacrylamide gel electrophoresis in combination with silver staining and by enzyme-linked immunosorbent assay showed 96.7% identical results in tests with 1,304 stool specimens from diarrheic patients. The polyacrylamide gel electrophoresis method can be modified to reduce cost and working time. Phenol extraction of stools, however, is essential in maintaining the sensitivity of the method.


J Clin Microbiol. 1988 January; 26(1): 158-160







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