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J Clin Microbiol. 1988 October; 26(10): 1962-1966

Coagglutination of Vibrio cholerae, Vibrio mimicus, and Vibrio vulnificus with anti-flagellar monoclonal antibody.

Department of Microbiology, Louisiana State University, Baton Rouge 70803.

ABSTRACT

Monoclonal antibodies (MAbs) with serological activity for purified flagellar (H) core protein prepared from Vibrio cholerae were identified by enzyme-linked immunosorbent assay. Four of these MAbs reacted with the flagella of V. cholerae and V. mimicus exclusively, while eight MAbs reacted with at least 1 of 30 heterologous Vibrio species tested by enzyme-linked immunosorbent assay or coagglutination. It appears that V. cholerae and V. mimicus express similar, if not identical, H determinants unique to these two Vibrio species. Staphylococcus aureus cells or latex beads armed with the four species-specific MAbs coagglutinated each of 47 isolates identified bacteriologically as V. cholerae or V. mimicus from among 103 Vibrio isolates tested. One coagglutination reagent armed with anti-V. vulnificus H MAb exhibited species specificity in that only V. vulnificus cells were coagglutinated from among the 31 Vibrio species examined. This reagent coagglutinated 20 isolates identified bacteriologically as V. vulnificus in a serological survey. MAb coagglutination reagents offer a rapid, specific, and economical alternative to the classical bacteriological approach to identify the human pathogens V. cholerae, V. mimicus, and V. vulnificus.

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• Datta, S., Janes, M. E., Simonson, J. G. (2008). Immunomagnetic Separation and Coagglutination of Vibrio parahaemolyticus with Anti-Flagellar Protein Monoclonal Antibody. CVI 15: 1541-1546 [Abstract] [Full Text]