This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lopez-Vidal, Y Articles by Svennerholm, A M Search for Related Content PubMed PubMed Citation Articles by Lopez-Vidal, Y Articles by Svennerholm, A M

Monoclonal antibodies against different epitopes on colonization factor antigen I of enterotoxin-producing Escherichia coli.

Department of Medical Microbiology, University of Göteborg, Sweden.

ABSTRACT

Hybridoma-secreting monoclonal antibodies (MAbs) against colonization factor antigen I (CFA/I) were produced by the fusion of spleen cells from immunized BALB/c mice with F/O myeloma cells. The 25 MAbs with the highest antibody titer against CFA/I, as determined by an enzyme-linked immunosorbent assay, were studied for specificity and the capacity to agglutinate CFA/I-carrying bacteria. Most of the MAbs agglutinated a majority of 16 CFA/I-positive strains tested but not any of a number of CFA-deficient mutants or strains carrying other colonization factors (CFA/II and CFA/IV). One MAb that agglutinated all the CFA/I-positive strains and one MAb that did not agglutinate any of these strains were compared for their reactivities with different preparations of CFA/I. Whereas both MAbs bound to or could be inhibited by isolated CFA/I fimbriae as well as the subunit protein, only the agglutinating MAb bound to CFA/I, as expressed on whole bacteria. The nonagglutinating MAb, on the other hand, bound considerably better than the agglutinating MAb to a peptide corresponding to the 46 N-terminal amino acid residues of the CFA/I subunit protein. These results suggest that the two MAbs are directed against different epitopes on CFA/I.

This article has been cited by other articles:

• Jansson, L., Tobias, J., Lebens, M., Svennerholm, A.-M., Teneberg, S. (2006). The Major Subunit, CfaB, of Colonization Factor Antigen I from Enterotoxigenic Escherichia coli Is a Glycosphingolipid Binding Protein.. Infect. Immun. 74: 3488-3497 [Abstract] [Full Text]  
• Anantha, R. P., McVeigh, A. L., Lee, L. H., Agnew, M. K., Cassels, F. J., Scott, D. A., Whittam, T. S., Savarino, S. J. (2004). Evolutionary and Functional Relationships of Colonization Factor Antigen I and Other Class 5 Adhesive Fimbriae of Enterotoxigenic Escherichia coli. Infect. Immun. 72: 7190-7201 [Abstract] [Full Text]  
• Sbrogio-Almeida, M. E., Mosca, T., Massis, L. M., Abrahamsohn, I. A., Ferreira, L. C. S. (2004). Host and Bacterial Factors Affecting Induction of Immune Responses to Flagellin Expressed by Attenuated Salmonella Vaccine Strains. Infect. Immun. 72: 2546-2555 [Abstract] [Full Text]  
• Qadri, F., Das, S. K., Faruque, A. S. G., Fuchs, G. J., Albert, M. J., Sack, R. B., Svennerholm, A.-M. (2000). Prevalence of Toxin Types and Colonization Factors in Enterotoxigenic Escherichia coli Isolated during a 2-Year Period from Diarrheal Patients in Bangladesh. J. Clin. Microbiol. 38: 27-31 [Abstract] [Full Text]  
• Helander, A., Wenneras, C., Qadri, F., Svennerholm, A.-M. (1998). Antibody Responses in Humans against Coli Surface Antigen 6 of Enterotoxigenic Escherichia coli. Infect. Immun. 66: 4507-4510 [Abstract] [Full Text]